TY - JOUR
T1 - The fission yeast Pvg1p has galactose-specific pyruvyltransferase activity
AU - Yoritsune, Ken Ichi
AU - Matsuzawa, Tomohiko
AU - Ohashi, Takao
AU - Takegawa, Kaoru
N1 - Funding Information:
We thank Drs. Ken Kitajima and Yuji Ise for valuable discussions. This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan (K.T.). T.M. was supported in part by the Japanese Society for the Promotion of Science (JSPS).
PY - 2013/4/2
Y1 - 2013/4/2
N2 - N-Glycan from the fission yeast Schizosaccharomyces pombe contains outer-chain pyruvic acid 4,6-ketal-linked galactose (PvGal). Here, we characterized a putative S. pombe pyruvyltransferase, Pvg1p, reported to be essential for biosynthesis of PvGal. When p-nitrophenyl-β-Gal (pNP-β-Gal) was used as a substrate, the structure of the recombinant Pvg1p product was determined to be pNP-PvGal by one- and two-dimensional NMR spectroscopy. The recombinant Pvg1p transferred pyruvyl residues from phosphoenolpyruvate specifically to β-linked galactose.
AB - N-Glycan from the fission yeast Schizosaccharomyces pombe contains outer-chain pyruvic acid 4,6-ketal-linked galactose (PvGal). Here, we characterized a putative S. pombe pyruvyltransferase, Pvg1p, reported to be essential for biosynthesis of PvGal. When p-nitrophenyl-β-Gal (pNP-β-Gal) was used as a substrate, the structure of the recombinant Pvg1p product was determined to be pNP-PvGal by one- and two-dimensional NMR spectroscopy. The recombinant Pvg1p transferred pyruvyl residues from phosphoenolpyruvate specifically to β-linked galactose.
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U2 - 10.1016/j.febslet.2013.02.016
DO - 10.1016/j.febslet.2013.02.016
M3 - Article
C2 - 23422075
AN - SCOPUS:84875265265
SN - 0014-5793
VL - 587
SP - 917
EP - 921
JO - FEBS Letters
JF - FEBS Letters
IS - 7
ER -