The formation of the chick ileal muscle layers as revealed by α-smooth muscle actin immunohistochemistry

Kouji Masumoto, Osami Nada, Sachiyo Suita, Tomoaki Taguchi, Rishu Guo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The genesis of intestinal smooth muscle layers was immunohistochemically investigated by use of an antibody to α-smooth muscle actin (α-SMA) in the developing chick ileum. Myoblast cells positive for α-SMA were already found in the presumptive circular muscle layer on E 8.5. On E 11.5 radially oriented muscle fibers were protruded from the outermost layer of the developing circular musculature and then formed a tuft-like aggregates. These radial muscle bundles were bent into an L-shape. The long distal extension of muscle bundles run parallel to the long axis of the ileal loop and developed into the longitudinal muscle layer. The obliquely oriented muscle fibers, locating at the intermuscular space of the muscularis propria, probably are to be considered a remnant of the short extension of radial muscle bundles. The muscularis mucosae was formed by the processes equivalent to the genesis of longitudinal muscle layer. On E 14.5 centripetally oriented muscle fibers emerged from the innermost layer of circular musculature. The long distal extension of centripetal fibers lay along the inner surface of developing circular musculature. On E 19.5 the longitudinal muscle layer of the muscularis mucosae was newly formed by separating from the circular musculature. The villous myoblast cells initially developed from the innermost layer of the muscularis mucosae on E 18.5, and were widely distributed in the lamina propria mucosae on E 20.5. Temporal and chronological pattern in expression of α-SMA was observed during the development of the chick intestinal smooth muscle. By E 14.5 the entire layer of the muscularis propria was intensely immunostained for α-SMA, but from E 15.5 onward the staining intensity gradually began to decrease from the outer half of the circular musculature. Finally, the immunoreactivity was localized in the inner layer of circular muscle and the longitudinal muscle layer. A possible functional role of this inner layer of circular muscle is discussed.

Original languageEnglish
Pages (from-to)121-129
Number of pages9
JournalAnatomy and Embryology
Volume201
Issue number2
DOIs
Publication statusPublished - Jan 1 2000

Fingerprint

Smooth Muscle
Actins
Immunohistochemistry
Muscles
Mucous Membrane
Myoblasts
Ileum
Staining and Labeling
Antibodies

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Embryology
  • Developmental Biology
  • Cell Biology

Cite this

The formation of the chick ileal muscle layers as revealed by α-smooth muscle actin immunohistochemistry. / Masumoto, Kouji; Nada, Osami; Suita, Sachiyo; Taguchi, Tomoaki; Guo, Rishu.

In: Anatomy and Embryology, Vol. 201, No. 2, 01.01.2000, p. 121-129.

Research output: Contribution to journalArticle

Masumoto, Kouji ; Nada, Osami ; Suita, Sachiyo ; Taguchi, Tomoaki ; Guo, Rishu. / The formation of the chick ileal muscle layers as revealed by α-smooth muscle actin immunohistochemistry. In: Anatomy and Embryology. 2000 ; Vol. 201, No. 2. pp. 121-129.
@article{b3b1e74f5d734375b1042ff2986ff0af,
title = "The formation of the chick ileal muscle layers as revealed by α-smooth muscle actin immunohistochemistry",
abstract = "The genesis of intestinal smooth muscle layers was immunohistochemically investigated by use of an antibody to α-smooth muscle actin (α-SMA) in the developing chick ileum. Myoblast cells positive for α-SMA were already found in the presumptive circular muscle layer on E 8.5. On E 11.5 radially oriented muscle fibers were protruded from the outermost layer of the developing circular musculature and then formed a tuft-like aggregates. These radial muscle bundles were bent into an L-shape. The long distal extension of muscle bundles run parallel to the long axis of the ileal loop and developed into the longitudinal muscle layer. The obliquely oriented muscle fibers, locating at the intermuscular space of the muscularis propria, probably are to be considered a remnant of the short extension of radial muscle bundles. The muscularis mucosae was formed by the processes equivalent to the genesis of longitudinal muscle layer. On E 14.5 centripetally oriented muscle fibers emerged from the innermost layer of circular musculature. The long distal extension of centripetal fibers lay along the inner surface of developing circular musculature. On E 19.5 the longitudinal muscle layer of the muscularis mucosae was newly formed by separating from the circular musculature. The villous myoblast cells initially developed from the innermost layer of the muscularis mucosae on E 18.5, and were widely distributed in the lamina propria mucosae on E 20.5. Temporal and chronological pattern in expression of α-SMA was observed during the development of the chick intestinal smooth muscle. By E 14.5 the entire layer of the muscularis propria was intensely immunostained for α-SMA, but from E 15.5 onward the staining intensity gradually began to decrease from the outer half of the circular musculature. Finally, the immunoreactivity was localized in the inner layer of circular muscle and the longitudinal muscle layer. A possible functional role of this inner layer of circular muscle is discussed.",
author = "Kouji Masumoto and Osami Nada and Sachiyo Suita and Tomoaki Taguchi and Rishu Guo",
year = "2000",
month = "1",
day = "1",
doi = "10.1007/PL00008232",
language = "English",
volume = "201",
pages = "121--129",
journal = "Anatomy and Embryology",
issn = "0340-2061",
number = "2",

}

TY - JOUR

T1 - The formation of the chick ileal muscle layers as revealed by α-smooth muscle actin immunohistochemistry

AU - Masumoto, Kouji

AU - Nada, Osami

AU - Suita, Sachiyo

AU - Taguchi, Tomoaki

AU - Guo, Rishu

PY - 2000/1/1

Y1 - 2000/1/1

N2 - The genesis of intestinal smooth muscle layers was immunohistochemically investigated by use of an antibody to α-smooth muscle actin (α-SMA) in the developing chick ileum. Myoblast cells positive for α-SMA were already found in the presumptive circular muscle layer on E 8.5. On E 11.5 radially oriented muscle fibers were protruded from the outermost layer of the developing circular musculature and then formed a tuft-like aggregates. These radial muscle bundles were bent into an L-shape. The long distal extension of muscle bundles run parallel to the long axis of the ileal loop and developed into the longitudinal muscle layer. The obliquely oriented muscle fibers, locating at the intermuscular space of the muscularis propria, probably are to be considered a remnant of the short extension of radial muscle bundles. The muscularis mucosae was formed by the processes equivalent to the genesis of longitudinal muscle layer. On E 14.5 centripetally oriented muscle fibers emerged from the innermost layer of circular musculature. The long distal extension of centripetal fibers lay along the inner surface of developing circular musculature. On E 19.5 the longitudinal muscle layer of the muscularis mucosae was newly formed by separating from the circular musculature. The villous myoblast cells initially developed from the innermost layer of the muscularis mucosae on E 18.5, and were widely distributed in the lamina propria mucosae on E 20.5. Temporal and chronological pattern in expression of α-SMA was observed during the development of the chick intestinal smooth muscle. By E 14.5 the entire layer of the muscularis propria was intensely immunostained for α-SMA, but from E 15.5 onward the staining intensity gradually began to decrease from the outer half of the circular musculature. Finally, the immunoreactivity was localized in the inner layer of circular muscle and the longitudinal muscle layer. A possible functional role of this inner layer of circular muscle is discussed.

AB - The genesis of intestinal smooth muscle layers was immunohistochemically investigated by use of an antibody to α-smooth muscle actin (α-SMA) in the developing chick ileum. Myoblast cells positive for α-SMA were already found in the presumptive circular muscle layer on E 8.5. On E 11.5 radially oriented muscle fibers were protruded from the outermost layer of the developing circular musculature and then formed a tuft-like aggregates. These radial muscle bundles were bent into an L-shape. The long distal extension of muscle bundles run parallel to the long axis of the ileal loop and developed into the longitudinal muscle layer. The obliquely oriented muscle fibers, locating at the intermuscular space of the muscularis propria, probably are to be considered a remnant of the short extension of radial muscle bundles. The muscularis mucosae was formed by the processes equivalent to the genesis of longitudinal muscle layer. On E 14.5 centripetally oriented muscle fibers emerged from the innermost layer of circular musculature. The long distal extension of centripetal fibers lay along the inner surface of developing circular musculature. On E 19.5 the longitudinal muscle layer of the muscularis mucosae was newly formed by separating from the circular musculature. The villous myoblast cells initially developed from the innermost layer of the muscularis mucosae on E 18.5, and were widely distributed in the lamina propria mucosae on E 20.5. Temporal and chronological pattern in expression of α-SMA was observed during the development of the chick intestinal smooth muscle. By E 14.5 the entire layer of the muscularis propria was intensely immunostained for α-SMA, but from E 15.5 onward the staining intensity gradually began to decrease from the outer half of the circular musculature. Finally, the immunoreactivity was localized in the inner layer of circular muscle and the longitudinal muscle layer. A possible functional role of this inner layer of circular muscle is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0033955333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033955333&partnerID=8YFLogxK

U2 - 10.1007/PL00008232

DO - 10.1007/PL00008232

M3 - Article

C2 - 10672364

AN - SCOPUS:0033955333

VL - 201

SP - 121

EP - 129

JO - Anatomy and Embryology

JF - Anatomy and Embryology

SN - 0340-2061

IS - 2

ER -