The gelatinase biosynthesis-activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing

Sean Littlewood, Helena Tattersall, Charlotte S. Hughes, Rohanah Hussain, Pikyee Ma, Stephen E. Harding, Jiro Nakayama, Mary K. Phillips-Jones

Research output: Contribution to journalArticle

Abstract

Quorum-sensing mechanisms regulate gene expression in response to changing cell-population density detected through pheromones. In Enterococcus faecalis, Fsr quorum sensing produces and responds to the gelatinase biosynthesis-activating pheromone (GBAP). Here we establish that the enterococcal FsrB membrane protein has a direct role connected with GBAP by showing that GBAP binds to purified FsrB. Far-UV CD measurements demonstrated a predominantly α-helical protein exhibiting a small level of conformational flexibility. Fivefold (400 μm) GBAP stabilised FsrB (80 μm) secondary structure. FsrB thermal denaturation in the presence and absence of GBAP revealed melting temperatures of 70.1 and 60.8 °C, respectively, demonstrating GBAP interactions and increased thermal stability conferred by GBAP. Addition of GBAP also resulted in tertiary structural changes, confirming GBAP binding.

Original languageEnglish
Pages (from-to)553-563
Number of pages11
JournalFEBS Letters
Volume594
Issue number3
DOIs
Publication statusPublished - Feb 1 2020

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Quorum Sensing
Membrane Proteins
Hot Temperature
gelatinase biosynthesis-activating pheromone
Denaturation
Pheromones
Population Density
Gene expression
Freezing
Melting point
Thermodynamic stability
Cell Count
Gene Expression
Temperature

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Littlewood, S., Tattersall, H., Hughes, C. S., Hussain, R., Ma, P., Harding, S. E., ... Phillips-Jones, M. K. (2020). The gelatinase biosynthesis-activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing. FEBS Letters, 594(3), 553-563. https://doi.org/10.1002/1873-3468.13634

The gelatinase biosynthesis-activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing. / Littlewood, Sean; Tattersall, Helena; Hughes, Charlotte S.; Hussain, Rohanah; Ma, Pikyee; Harding, Stephen E.; Nakayama, Jiro; Phillips-Jones, Mary K.

In: FEBS Letters, Vol. 594, No. 3, 01.02.2020, p. 553-563.

Research output: Contribution to journalArticle

Littlewood, S, Tattersall, H, Hughes, CS, Hussain, R, Ma, P, Harding, SE, Nakayama, J & Phillips-Jones, MK 2020, 'The gelatinase biosynthesis-activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing', FEBS Letters, vol. 594, no. 3, pp. 553-563. https://doi.org/10.1002/1873-3468.13634
Littlewood, Sean ; Tattersall, Helena ; Hughes, Charlotte S. ; Hussain, Rohanah ; Ma, Pikyee ; Harding, Stephen E. ; Nakayama, Jiro ; Phillips-Jones, Mary K. / The gelatinase biosynthesis-activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing. In: FEBS Letters. 2020 ; Vol. 594, No. 3. pp. 553-563.
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