The GINS complex from the thermophilic archaeon, Thermoplasma acidophilum may function as a homotetramer in DNA replication

Hiromi Ogino, Sonoko Ishino, Kouta Mayanagi, Gyri Teien Haugland, Nils Kåre Birkeland, Akihiko Yamagishi, Yoshizumi Ishino

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The eukaryotic GINS heterotetramer, consisting of Sld5, Psf1, Psf2, and Psf3, participates in "CMG complex" formation with mini-chromosome maintenance (MCM) and Cdc45 as a key component of a replicative helicase. There are only two homologs of the GINS proteins in Archaea, and these proteins, Gins51 and Gins23, form a heterotetrameric GINS with a 2:2 molar ratio. The Pyrococcus furiosus GINS stimulates the ATPase and helicase activities of its cognate MCM, whereas the Sulfolobus solfataricus GINS does not affect those activities of its cognate MCM, although the proteins bind each other. Intriguingly, Thermoplasma acidophilum, as well as many euryarchaea, have only one gene encoding the sequence homologous to that of archaeal Gins protein (Gins51) on the genome. In this study, we investigated the biochemical properties of the gene product (TaGins51). A gel filtration and electron microscopy revealed that TaGins51 forms a homotetramer. A physical interaction between TaGins51 and TaMcm was detected by a surface plasmon resonance analysis. Unexpectedly, TaGins51 inhibited the ATPase activity, but did not affect the helicase activity of its cognate MCM. These results suggest that another factor is required to form a stable helicase complex with MCM and GINS at the replication fork in T. acidophilum cells.

Original languageEnglish
Pages (from-to)529-539
Number of pages11
JournalExtremophiles
Volume15
Issue number4
DOIs
Publication statusPublished - Jul 1 2011

Fingerprint

Thermoplasma
Archaea
DNA Replication
Chromosomes
Maintenance
Adenosine Triphosphatases
Archaeal Proteins
Pyrococcus furiosus
Sulfolobus solfataricus
Proteins
Surface Plasmon Resonance
Sequence Homology
Genes
Gel Chromatography
Electron Microscopy
Genome

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Medicine

Cite this

The GINS complex from the thermophilic archaeon, Thermoplasma acidophilum may function as a homotetramer in DNA replication. / Ogino, Hiromi; Ishino, Sonoko; Mayanagi, Kouta; Haugland, Gyri Teien; Birkeland, Nils Kåre; Yamagishi, Akihiko; Ishino, Yoshizumi.

In: Extremophiles, Vol. 15, No. 4, 01.07.2011, p. 529-539.

Research output: Contribution to journalArticle

Ogino, Hiromi ; Ishino, Sonoko ; Mayanagi, Kouta ; Haugland, Gyri Teien ; Birkeland, Nils Kåre ; Yamagishi, Akihiko ; Ishino, Yoshizumi. / The GINS complex from the thermophilic archaeon, Thermoplasma acidophilum may function as a homotetramer in DNA replication. In: Extremophiles. 2011 ; Vol. 15, No. 4. pp. 529-539.
@article{39009d537911436083d540a53d7be3f5,
title = "The GINS complex from the thermophilic archaeon, Thermoplasma acidophilum may function as a homotetramer in DNA replication",
abstract = "The eukaryotic GINS heterotetramer, consisting of Sld5, Psf1, Psf2, and Psf3, participates in {"}CMG complex{"} formation with mini-chromosome maintenance (MCM) and Cdc45 as a key component of a replicative helicase. There are only two homologs of the GINS proteins in Archaea, and these proteins, Gins51 and Gins23, form a heterotetrameric GINS with a 2:2 molar ratio. The Pyrococcus furiosus GINS stimulates the ATPase and helicase activities of its cognate MCM, whereas the Sulfolobus solfataricus GINS does not affect those activities of its cognate MCM, although the proteins bind each other. Intriguingly, Thermoplasma acidophilum, as well as many euryarchaea, have only one gene encoding the sequence homologous to that of archaeal Gins protein (Gins51) on the genome. In this study, we investigated the biochemical properties of the gene product (TaGins51). A gel filtration and electron microscopy revealed that TaGins51 forms a homotetramer. A physical interaction between TaGins51 and TaMcm was detected by a surface plasmon resonance analysis. Unexpectedly, TaGins51 inhibited the ATPase activity, but did not affect the helicase activity of its cognate MCM. These results suggest that another factor is required to form a stable helicase complex with MCM and GINS at the replication fork in T. acidophilum cells.",
author = "Hiromi Ogino and Sonoko Ishino and Kouta Mayanagi and Haugland, {Gyri Teien} and Birkeland, {Nils K{\aa}re} and Akihiko Yamagishi and Yoshizumi Ishino",
year = "2011",
month = "7",
day = "1",
doi = "10.1007/s00792-011-0383-2",
language = "English",
volume = "15",
pages = "529--539",
journal = "Extremophiles",
issn = "1431-0651",
publisher = "Springer Japan",
number = "4",

}

TY - JOUR

T1 - The GINS complex from the thermophilic archaeon, Thermoplasma acidophilum may function as a homotetramer in DNA replication

AU - Ogino, Hiromi

AU - Ishino, Sonoko

AU - Mayanagi, Kouta

AU - Haugland, Gyri Teien

AU - Birkeland, Nils Kåre

AU - Yamagishi, Akihiko

AU - Ishino, Yoshizumi

PY - 2011/7/1

Y1 - 2011/7/1

N2 - The eukaryotic GINS heterotetramer, consisting of Sld5, Psf1, Psf2, and Psf3, participates in "CMG complex" formation with mini-chromosome maintenance (MCM) and Cdc45 as a key component of a replicative helicase. There are only two homologs of the GINS proteins in Archaea, and these proteins, Gins51 and Gins23, form a heterotetrameric GINS with a 2:2 molar ratio. The Pyrococcus furiosus GINS stimulates the ATPase and helicase activities of its cognate MCM, whereas the Sulfolobus solfataricus GINS does not affect those activities of its cognate MCM, although the proteins bind each other. Intriguingly, Thermoplasma acidophilum, as well as many euryarchaea, have only one gene encoding the sequence homologous to that of archaeal Gins protein (Gins51) on the genome. In this study, we investigated the biochemical properties of the gene product (TaGins51). A gel filtration and electron microscopy revealed that TaGins51 forms a homotetramer. A physical interaction between TaGins51 and TaMcm was detected by a surface plasmon resonance analysis. Unexpectedly, TaGins51 inhibited the ATPase activity, but did not affect the helicase activity of its cognate MCM. These results suggest that another factor is required to form a stable helicase complex with MCM and GINS at the replication fork in T. acidophilum cells.

AB - The eukaryotic GINS heterotetramer, consisting of Sld5, Psf1, Psf2, and Psf3, participates in "CMG complex" formation with mini-chromosome maintenance (MCM) and Cdc45 as a key component of a replicative helicase. There are only two homologs of the GINS proteins in Archaea, and these proteins, Gins51 and Gins23, form a heterotetrameric GINS with a 2:2 molar ratio. The Pyrococcus furiosus GINS stimulates the ATPase and helicase activities of its cognate MCM, whereas the Sulfolobus solfataricus GINS does not affect those activities of its cognate MCM, although the proteins bind each other. Intriguingly, Thermoplasma acidophilum, as well as many euryarchaea, have only one gene encoding the sequence homologous to that of archaeal Gins protein (Gins51) on the genome. In this study, we investigated the biochemical properties of the gene product (TaGins51). A gel filtration and electron microscopy revealed that TaGins51 forms a homotetramer. A physical interaction between TaGins51 and TaMcm was detected by a surface plasmon resonance analysis. Unexpectedly, TaGins51 inhibited the ATPase activity, but did not affect the helicase activity of its cognate MCM. These results suggest that another factor is required to form a stable helicase complex with MCM and GINS at the replication fork in T. acidophilum cells.

UR - http://www.scopus.com/inward/record.url?scp=79959355742&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959355742&partnerID=8YFLogxK

U2 - 10.1007/s00792-011-0383-2

DO - 10.1007/s00792-011-0383-2

M3 - Article

C2 - 21656171

AN - SCOPUS:79959355742

VL - 15

SP - 529

EP - 539

JO - Extremophiles

JF - Extremophiles

SN - 1431-0651

IS - 4

ER -