TY - JOUR
T1 - The gnd gene encoding a novel 6-phosphogluconate dehydrogenase and its adjacent region of Actinobacillus actinomycetemcomitans chromosomal DNA
AU - Yoshida, Yasuo
AU - Nakano, Yoshio
AU - Yamashita, Yoshihisa
AU - Koga, Toshihiko
N1 - Funding Information:
We thank Dr. Richard E. Wolf, Jr. for providing us the gnd0 and zwf0 strain of E. coli. This work was supported in part by Grants-in-Aid for Scienti®c Research 07557136 and 08457572 from the Ministry of Education, Science, Sports, and Culture, Tokyo, Japan, and by a research grant from the Funds for Comprehensive Research on Aging and Health.
PY - 1997/1/3
Y1 - 1997/1/3
N2 - A 10-kb DNA fragment containing the gnd gene from Actinobacillus actinomy-cetemcomitans Y4 was isolated and sequenced. The structural gnd gene codes for 6-phosphogluconate dehydrogenase that consists of 484 amino acids. In contrast to the gnd gene in Escherichia coli, Salmonella typhimurium, or Klebsiella pneumoniae, the gnd gene of A. actinomycetemcomitans was not located in the rfb or cps operon. The zwf gene encoding glucose 6-phosphate dehydrogenase, which is another enzyme consisting of pentose-phosphate pathway, sided at 3.8-kb upstream from the gnd gene. A phylogenetic tree based on sequence analyses showed higher homology of 6-phospho-gluconate dehydrogenase of A. actinomycetemcomitans with the eucaryotic enzymes rather than with bacterial enzymes.
AB - A 10-kb DNA fragment containing the gnd gene from Actinobacillus actinomy-cetemcomitans Y4 was isolated and sequenced. The structural gnd gene codes for 6-phosphogluconate dehydrogenase that consists of 484 amino acids. In contrast to the gnd gene in Escherichia coli, Salmonella typhimurium, or Klebsiella pneumoniae, the gnd gene of A. actinomycetemcomitans was not located in the rfb or cps operon. The zwf gene encoding glucose 6-phosphate dehydrogenase, which is another enzyme consisting of pentose-phosphate pathway, sided at 3.8-kb upstream from the gnd gene. A phylogenetic tree based on sequence analyses showed higher homology of 6-phospho-gluconate dehydrogenase of A. actinomycetemcomitans with the eucaryotic enzymes rather than with bacterial enzymes.
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U2 - 10.1006/bbrc.1996.5917
DO - 10.1006/bbrc.1996.5917
M3 - Article
C2 - 9020051
AN - SCOPUS:0031550264
SN - 0006-291X
VL - 230
SP - 220
EP - 225
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -