TY - JOUR
T1 - The groESL operon of the halophilic lactic acid bacterium tetragenococcus halophila
AU - Fukuda, Daisuke
AU - Watanabe, Maki
AU - Aso, Yuji
AU - Sonomoto, Kenji
AU - Ishizaki, Ayaaki
N1 - Funding Information:
This work was partly supported by the Sasakawa Scientiˆc Research Grant from The Japan Science Society.
PY - 2002
Y1 - 2002
N2 - The groESL operon of the halophilic lactic acid bacterium Tetragenococcus halophila was cloned by a PCR-based method. The molecular masses of GroES and GroEL proteins were calculated to be 10,153 and 56,893 Da, respectively. The amount of groESL mRNA was increased 3.8-fold by heat shock (45°C), and 4-fold by high NaCl (3-4 M). The Bacillus subtilis σA-like constitutive promoter existed in front of groES, and was used under both normal and stress (heat shock and high salinity) conditions.
AB - The groESL operon of the halophilic lactic acid bacterium Tetragenococcus halophila was cloned by a PCR-based method. The molecular masses of GroES and GroEL proteins were calculated to be 10,153 and 56,893 Da, respectively. The amount of groESL mRNA was increased 3.8-fold by heat shock (45°C), and 4-fold by high NaCl (3-4 M). The Bacillus subtilis σA-like constitutive promoter existed in front of groES, and was used under both normal and stress (heat shock and high salinity) conditions.
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U2 - 10.1271/bbb.66.1176
DO - 10.1271/bbb.66.1176
M3 - Article
C2 - 12092841
AN - SCOPUS:0036562589
SN - 0916-8451
VL - 66
SP - 1176
EP - 1180
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 5
ER -