The groESL operon of the halophilic lactic acid bacterium tetragenococcus halophila

Daisuke Fukuda, Maki Watanabe, Yuji Aso, Kenji Sonomoto, Ayaaki Ishizaki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The groESL operon of the halophilic lactic acid bacterium Tetragenococcus halophila was cloned by a PCR-based method. The molecular masses of GroES and GroEL proteins were calculated to be 10,153 and 56,893 Da, respectively. The amount of groESL mRNA was increased 3.8-fold by heat shock (45°C), and 4-fold by high NaCl (3-4 M). The Bacillus subtilis σA-like constitutive promoter existed in front of groES, and was used under both normal and stress (heat shock and high salinity) conditions.

Original languageEnglish
Pages (from-to)1176-1180
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number5
DOIs
Publication statusPublished - Jan 1 2002

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Enterococcaceae
Operon
Shock
Lactic Acid
Bacteria
Chaperonin 10
Hot Temperature
Chaperonin 60
Salinity
Molecular mass
Bacilli
Bacillus subtilis
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

The groESL operon of the halophilic lactic acid bacterium tetragenococcus halophila. / Fukuda, Daisuke; Watanabe, Maki; Aso, Yuji; Sonomoto, Kenji; Ishizaki, Ayaaki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 66, No. 5, 01.01.2002, p. 1176-1180.

Research output: Contribution to journalArticle

Fukuda, Daisuke ; Watanabe, Maki ; Aso, Yuji ; Sonomoto, Kenji ; Ishizaki, Ayaaki. / The groESL operon of the halophilic lactic acid bacterium tetragenococcus halophila. In: Bioscience, Biotechnology and Biochemistry. 2002 ; Vol. 66, No. 5. pp. 1176-1180.
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