The growth-promoting activity of egg white proteins in the C2C12 myoblast cell line

Wataru Mizunoya, Ayumi Tashima, Yusuke Sato, Ryuichi Tatsumi, Yoshihide Ikeuchi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, we examined the effects of several egg white proteins (ovalbumin, ovomucoid, ovotransferrin and lysozyme) on proliferation and myotube growth in C2C12 murine myoblast cells. Cell proliferation was measured using a water-soluble tetrazolium salt (WST-8)-based assay and then validated using Giemsa staining. Significant proliferative activities of C2C12 cells were observed in response to the addition of 10(-5) -10(-4) mol/L ovalbumin or ovomucoid. Ovotransferrin decreased C2C12 cell proliferation and lysozyme showed no significant effects on the proliferation of C2C12 cells. In contrast, the proliferative effects of ovalbumin and ovomucoid were not observed in 3T3-L1 murine preadipocyte cells. We also measured the effects of ovalbumin and ovomucoid on C2C12 myotube diameters by using histological analysis. In comparison to control cells, myotube diameters were significantly increased in cells cultured in 10(-6) -10(-4) mol/L ovalbumin or ovomucoid, suggesting that ovalbumin and ovomucoid stimulate the growth of myotubes. Thus, our results clearly demonstrated that ovalbumin or ovomucoid stimulated the proliferation of myoblasts and growth of myotubes.

Original languageEnglish
Pages (from-to)194-199
Number of pages6
JournalAnimal science journal = Nihon chikusan Gakkaihō
Volume86
Issue number2
DOIs
Publication statusPublished - Feb 1 2015

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Ovomucin
Egg Proteins
myoblasts
Myoblasts
ovalbumin
Ovalbumin
egg albumen
Skeletal Muscle Fibers
cell lines
Cell Line
Conalbumin
proteins
cell proliferation
Cell Proliferation
Muramidase
lysozyme
Growth
Tetrazolium Salts
tetrazolium
mice

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

The growth-promoting activity of egg white proteins in the C2C12 myoblast cell line. / Mizunoya, Wataru; Tashima, Ayumi; Sato, Yusuke; Tatsumi, Ryuichi; Ikeuchi, Yoshihide.

In: Animal science journal = Nihon chikusan Gakkaihō, Vol. 86, No. 2, 01.02.2015, p. 194-199.

Research output: Contribution to journalArticle

Mizunoya, Wataru ; Tashima, Ayumi ; Sato, Yusuke ; Tatsumi, Ryuichi ; Ikeuchi, Yoshihide. / The growth-promoting activity of egg white proteins in the C2C12 myoblast cell line. In: Animal science journal = Nihon chikusan Gakkaihō. 2015 ; Vol. 86, No. 2. pp. 194-199.
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