The Hedgehog inhibitor suppresses the function of monocyte-derived dendritic cells from patients with advanced cancer under hypoxia

Hideya Ohnishi, Takashi Morisaki, Akifumi Kiyota, Norihiro Koya, Hiroto Tanaka, Masayo Umebayashi, Mitsuo Katano

Research output: Contribution to journalArticle

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Abstract

Immunotherapy using monocyte derived dendritic cells (Mo-DCs) from cancer patients has been developed; however, the Mo-DCs regularly studied have been derived from non-cancer bearing donors or mice, and evaluated in normoxic conditions. In the present study, we investigated the effects of Hedgehog (Hh) inhibitors which are being developed as molecular target drugs for cancer on the functions of Mo-DCs derived from patients with advanced cancer when cultured in a tumor-like hypoxic environment. Mo-DC induction, migration, chemotaxis, phagocytosis, maturation, IL-12 p40 or p70 secretion and the allogeneic lymphocyte stimulation activity of Mo-DCs from patients with advanced cancer were all significantly inhibited by the Hh inhibitor, cyclopamine under hypoxic conditions. Our results suggest that Hh signaling plays an important role in the maintenance and function of Mo-DCs derived from patients with advanced cancer when cultured under hypoxic conditions.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume436
Issue number1
DOIs
Publication statusPublished - Jun 21 2013

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Hedgehogs
Dendritic Cells
Monocytes
Neoplasms
Bearings (structural)
Lymphocytes
Interleukin-12
Chemotaxis
Lymphocyte Activation
Tumors
Phagocytosis
Immunotherapy
Hypoxia
Maintenance
Tissue Donors
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The Hedgehog inhibitor suppresses the function of monocyte-derived dendritic cells from patients with advanced cancer under hypoxia. / Ohnishi, Hideya; Morisaki, Takashi; Kiyota, Akifumi; Koya, Norihiro; Tanaka, Hiroto; Umebayashi, Masayo; Katano, Mitsuo.

In: Biochemical and Biophysical Research Communications, Vol. 436, No. 1, 21.06.2013, p. 53-59.

Research output: Contribution to journalArticle

Ohnishi, Hideya ; Morisaki, Takashi ; Kiyota, Akifumi ; Koya, Norihiro ; Tanaka, Hiroto ; Umebayashi, Masayo ; Katano, Mitsuo. / The Hedgehog inhibitor suppresses the function of monocyte-derived dendritic cells from patients with advanced cancer under hypoxia. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 436, No. 1. pp. 53-59.
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