The Human CCG1 Gene, Essential for Progression of the G1 Phase, Encodes a 210-Kilodalton Nuclear DNA-Binding Protein

Takeshi Sekiguchi, Yukiko Nohiro, Yasuhara Nakamura, Naoki Hisamoto, Takeharu Nishimoto

Research output: Contribution to journalArticlepeer-review

111 Citations (Scopus)

Abstract

The human CCG1 gene complements tsBN462, a temperature-sensitive G1 mutant of the BHK21 cell line. The previously cloned cDNA turned out to be a truncated form of the actual CCG1 cDNA. The newly cloned CCG1 cDNA was 6.0 kb and encoded a protein with a molecular mass of 210 kDa. Using an antibody to a predicted peptide from the CCG1 protein, a protein with a molecular mass of over 200 kDa was identified in human, monkey, and hamster cell lines. In the newly defined C-terminal region, an acidic domain was found. It contained four consensus target sequences for casein kinase II and was phosphorylated by this enzyme in vitro. However, this C-terminal region was not required to complement tsBN462 mutation since the region encoding the C-terminal part was frequently missing in complemented clones derived by DNA-mediated gene transfer. CCG1 contains a sequence similar to the putative DNA-binding domain of HMG1 in addition to the previously detected amino acid sequences common in nuclear proteins, such as a proline cluster and a nuclear translocation signal. Consistent with these predictions, CCG1 was present in nuclei, possessed DNA-binding activity, and was eluted with similar concentrations of salt, 0.3 to 0.4 M NaCl either from isolated nuclei or from a DNA-cellulose column.

Original languageEnglish
Pages (from-to)3317-3325
Number of pages9
JournalMolecular and cellular biology
Volume11
Issue number6
DOIs
Publication statusPublished - Jun 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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