The human endothelin-B receptor gene. Structural organization and chromosomal assignment

H. Arai, K. Nakao, K. Takaya, K. Hosoda, Y. Ogawa, S. Nakanishi, H. Imura

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

The gene encoding the human endothelin-B receptor (hET-BR) has been isolated, and its structural organization and chromosomal assignment have been determined. Southern blot analysis demonstrated a single copy of the hET-BR gene in the human genome. The hET-BR gene spans 24 kilobases and consists of seven exons and six introns. The size range for exons is 109- 2855 base pairs, although that for introns is 0.2-14.5 kilobases. Every intron occurs near the border of the putative transmembrane domain in the coding region. The major transcription initiation sites were mapped to the positions 258 and 229 base pairs upstream of the ATG initiation codon by primer extension and nuclease S1 protection experiments. The 5'-flanking region of the hET-BR gene lacks conventional TATA and CCAAT boxes but contains a sequence of potential Sp1 binding sites upstream of the transcription initiation sites. There are some canonical consensus sequences of cis-elements including GATA motif, acute phase reactant regulatory element, and E box. Using human-rodent somatic hybrid cell lines, the hET-BR gene was assigned to human chromosome 13. The present study will lead to a better understanding of the mechanism for the transcriptional regulation of the hET-BR gene and will give a clue as to how to search for possible genetic disorders of hET-BR.

Original languageEnglish
Pages (from-to)3463-3470
Number of pages8
JournalJournal of Biological Chemistry
Volume268
Issue number5
Publication statusPublished - Jan 1 1993

Fingerprint

Endothelin B Receptors
Genes
Introns
Transcription Initiation Site
Exons
Base Pairing
E-Box Elements
Gene encoding
Acute-Phase Proteins
5' Flanking Region
Chromosomes, Human, Pair 13
TATA Box
Inborn Genetic Diseases
Chromosomes
Initiator Codon
Consensus Sequence
Human Chromosomes
Human Genome
Southern Blotting
Binding Sites

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Arai, H., Nakao, K., Takaya, K., Hosoda, K., Ogawa, Y., Nakanishi, S., & Imura, H. (1993). The human endothelin-B receptor gene. Structural organization and chromosomal assignment. Journal of Biological Chemistry, 268(5), 3463-3470.

The human endothelin-B receptor gene. Structural organization and chromosomal assignment. / Arai, H.; Nakao, K.; Takaya, K.; Hosoda, K.; Ogawa, Y.; Nakanishi, S.; Imura, H.

In: Journal of Biological Chemistry, Vol. 268, No. 5, 01.01.1993, p. 3463-3470.

Research output: Contribution to journalArticle

Arai, H, Nakao, K, Takaya, K, Hosoda, K, Ogawa, Y, Nakanishi, S & Imura, H 1993, 'The human endothelin-B receptor gene. Structural organization and chromosomal assignment', Journal of Biological Chemistry, vol. 268, no. 5, pp. 3463-3470.
Arai H, Nakao K, Takaya K, Hosoda K, Ogawa Y, Nakanishi S et al. The human endothelin-B receptor gene. Structural organization and chromosomal assignment. Journal of Biological Chemistry. 1993 Jan 1;268(5):3463-3470.
Arai, H. ; Nakao, K. ; Takaya, K. ; Hosoda, K. ; Ogawa, Y. ; Nakanishi, S. ; Imura, H. / The human endothelin-B receptor gene. Structural organization and chromosomal assignment. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 5. pp. 3463-3470.
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