The hydroxyl radical scavenger MCI-186 protects the liver from experimental cold ischaemia-reperfusion injury

M. Ninomiya, M. Shimada, Noboru Harada, Yuji Soejima, T. Suehiro, Yoshihiko Maehara

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: Oxidative stress contributes to hepatic ischaemia-reperfusion (IR) injury in a biphasic pattern. In addition to direct cytotoxic effects, oxidative stress also initiates the signal transduction processes that promote second-phase liver injury. The present study investigated the effects of the hydroxyl radical scavenger MCI-186 on the biphasic process of hepatic cold IR injury. Methods: After cold preservation for 16 h, rat livers were reperfused on an isolated liver perfusion system for 120 min with oxygenated Krebs-Henseleit bicarbonate buffer. Perfusate samples were obtained serially, and portal flow rates were also recorded. To determine whether MCI-186 affected cytokine levels that control the second-phase injury, levels of interleukin (IL) 10 and tumour necrosis factor (TNF) α were measured in the perfusate. Results: Addition of MCI-186 1 mg/l into the perfusate significantly improved portal flow (P < 0.050), hepatic enzyme release into the perfusate (P = 0.038), total bile production (P = 0.029) and malondialdehyde concentration (P = 0.038). Furthermore, treatment with MCI-186 led to a substantial increase in IL-10 release (P = 0.032). TNF-α levels were not affected. Conclusions: MCI-186, an agent ready for clinical use, appears to have direct and indirect protective effects against hepatic cold IR injury.

Original languageEnglish
Pages (from-to)184-190
Number of pages7
JournalBritish Journal of Surgery
Volume91
Issue number2
DOIs
Publication statusPublished - Feb 1 2004

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Cold Ischemia
Reperfusion Injury
Hydroxyl Radical
Liver
Interleukin-10
Oxidative Stress
Tumor Necrosis Factor-alpha
Wounds and Injuries
Bicarbonates
phenylmethylpyrazolone
Malondialdehyde
Bile
Signal Transduction
Perfusion
Cytokines
Enzymes

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

The hydroxyl radical scavenger MCI-186 protects the liver from experimental cold ischaemia-reperfusion injury. / Ninomiya, M.; Shimada, M.; Harada, Noboru; Soejima, Yuji; Suehiro, T.; Maehara, Yoshihiko.

In: British Journal of Surgery, Vol. 91, No. 2, 01.02.2004, p. 184-190.

Research output: Contribution to journalArticle

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