The immune responses of common carp, Cyprinus carpio L., injected with carp interleukin-1β gene

Tomoya Kono, Kazuhiro Fujiki, Miki Nakao, Tomoki Yano, Makoto Endo, Masahiro Sakai

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The function of the common carp, Cyprinus carpio L., interleukin-1β (IL-1β) gene was studied by DNA injection. To investigate the immune responses to IL-1β, a plasmid construct of cytomegalovirus (CMV)-driven carp IL-1β was injected into the epaxial muscle of carp. IL-1β protein expressed in serum on 1, 3, and 5 days after plasmid injection was quantified by ELISA and immunohistochemistry. IL1-β gene injection increased proliferation of the lymphocytes by phytohemagglutinin (PHA). Macrophage functions, such as production of superoxide anion and phagocytosis, also were stimulated by IL-1β gene injection. Moreover, an increase in resistance to Aeromonas hydrophila infection was recorded in IL-1β-injected fish compared with control fish. Thus, the cloned homolog of IL-1β from carp has all the functional similarities to the mammalian IL-1β gene.

Original languageEnglish
Pages (from-to)413-419
Number of pages7
JournalJournal of Interferon and Cytokine Research
Volume22
Issue number4
DOIs
Publication statusPublished - Jan 1 2002

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cell Biology
  • Virology

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