The impact of pegylated-interferon α-2b on partial and massive hepatectomy model in rats

Hiroki Mori, Mitsuo Shimada, Toru Ikegami, Tohru Utsunomiya, Satoru Imura, Yuji Morine, Tetsuya Ikemoto, Jun Hanaoka, Shuichi Iwahashi, Yu Saito, Michihito Asanoma, Shinichiro Yamada, Hidenori Miyake

Research output: Contribution to journalArticle

Abstract

Background/Aims: The impact of pegylated-interferon (PEG-IFN) α-2b on liver regeneration has not yet been elucidated. Methodology: Rats were divided into the following four groups: 70% hepatectomy (Hx); 70% Hx+PEG-IFN; 90% Hx and 90% Hx+PEG-IFN group (n=6 each). Rats were pretreated with subcutaneous of PEG-IFN αa-2b (1.5μg/kg) administration 24 hours before Hx. Samples were taken 24, 48 and 72 hours after Hx and the following parameters were investigated: blood analysis (AST, WBC, PLT); liver weight to body weight ratio (Lw/Bw ratio); survival and PCNA labeling index (LI). Results: In the 90% Hx model, there was no significant difference between the Hx+PEG-IFN group and the Hx alone group in blood analysis; AST after postoperative 24 hours (2511 vs. 2466IU/L), WBC (1200 vs. 1290) and PLT (107 vs. 111 × 10 4/mm3), in Lw/Bw ratio at postoperative 0, 24, 48, 72 hours, respectively (0.38, 0.60, 1.14, 1.69 vs. 0.37, 0.64, 1.12, 1.63), in postoperative survival (40% vs. 45%), and in PCNA LI at postoperative 0, 24, 48, 72 hours, respectively (10.4%, 16.8%, 14.6%, 12.8% vs. 10.0%, 17.1%, 15.6%, 13.7%). In the 70% Hx model, there was no significant difference between the Hx+PEG-IFN group and the Hx alone group for all parameters. Conclusions: Our data demonstrated that PEG-IFN α-2b did not affect liver regeneration and the early use of PEG-IFN α-2b would cause no problems after liver transplantation using partial grafts including living donor liver transplantation.

Original languageEnglish
Pages (from-to)2300-2304
Number of pages5
JournalHepato-Gastroenterology
Volume59
Issue number119
DOIs
Publication statusPublished - Oct 2012
Externally publishedYes

Fingerprint

Hepatectomy
Interferons
Liver Regeneration
Proliferating Cell Nuclear Antigen
Liver Transplantation
Body Weight
Weights and Measures
Living Donors
Liver
Blood Group Antigens
Transplants

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Mori, H., Shimada, M., Ikegami, T., Utsunomiya, T., Imura, S., Morine, Y., ... Miyake, H. (2012). The impact of pegylated-interferon α-2b on partial and massive hepatectomy model in rats. Hepato-Gastroenterology, 59(119), 2300-2304. https://doi.org/10.5754/hge10401

The impact of pegylated-interferon α-2b on partial and massive hepatectomy model in rats. / Mori, Hiroki; Shimada, Mitsuo; Ikegami, Toru; Utsunomiya, Tohru; Imura, Satoru; Morine, Yuji; Ikemoto, Tetsuya; Hanaoka, Jun; Iwahashi, Shuichi; Saito, Yu; Asanoma, Michihito; Yamada, Shinichiro; Miyake, Hidenori.

In: Hepato-Gastroenterology, Vol. 59, No. 119, 10.2012, p. 2300-2304.

Research output: Contribution to journalArticle

Mori, H, Shimada, M, Ikegami, T, Utsunomiya, T, Imura, S, Morine, Y, Ikemoto, T, Hanaoka, J, Iwahashi, S, Saito, Y, Asanoma, M, Yamada, S & Miyake, H 2012, 'The impact of pegylated-interferon α-2b on partial and massive hepatectomy model in rats', Hepato-Gastroenterology, vol. 59, no. 119, pp. 2300-2304. https://doi.org/10.5754/hge10401
Mori, Hiroki ; Shimada, Mitsuo ; Ikegami, Toru ; Utsunomiya, Tohru ; Imura, Satoru ; Morine, Yuji ; Ikemoto, Tetsuya ; Hanaoka, Jun ; Iwahashi, Shuichi ; Saito, Yu ; Asanoma, Michihito ; Yamada, Shinichiro ; Miyake, Hidenori. / The impact of pegylated-interferon α-2b on partial and massive hepatectomy model in rats. In: Hepato-Gastroenterology. 2012 ; Vol. 59, No. 119. pp. 2300-2304.
@article{e84e888aa4644b3995b67d0de1644e20,
title = "The impact of pegylated-interferon α-2b on partial and massive hepatectomy model in rats",
abstract = "Background/Aims: The impact of pegylated-interferon (PEG-IFN) α-2b on liver regeneration has not yet been elucidated. Methodology: Rats were divided into the following four groups: 70{\%} hepatectomy (Hx); 70{\%} Hx+PEG-IFN; 90{\%} Hx and 90{\%} Hx+PEG-IFN group (n=6 each). Rats were pretreated with subcutaneous of PEG-IFN αa-2b (1.5μg/kg) administration 24 hours before Hx. Samples were taken 24, 48 and 72 hours after Hx and the following parameters were investigated: blood analysis (AST, WBC, PLT); liver weight to body weight ratio (Lw/Bw ratio); survival and PCNA labeling index (LI). Results: In the 90{\%} Hx model, there was no significant difference between the Hx+PEG-IFN group and the Hx alone group in blood analysis; AST after postoperative 24 hours (2511 vs. 2466IU/L), WBC (1200 vs. 1290) and PLT (107 vs. 111 × 10 4/mm3), in Lw/Bw ratio at postoperative 0, 24, 48, 72 hours, respectively (0.38, 0.60, 1.14, 1.69 vs. 0.37, 0.64, 1.12, 1.63), in postoperative survival (40{\%} vs. 45{\%}), and in PCNA LI at postoperative 0, 24, 48, 72 hours, respectively (10.4{\%}, 16.8{\%}, 14.6{\%}, 12.8{\%} vs. 10.0{\%}, 17.1{\%}, 15.6{\%}, 13.7{\%}). In the 70{\%} Hx model, there was no significant difference between the Hx+PEG-IFN group and the Hx alone group for all parameters. Conclusions: Our data demonstrated that PEG-IFN α-2b did not affect liver regeneration and the early use of PEG-IFN α-2b would cause no problems after liver transplantation using partial grafts including living donor liver transplantation.",
author = "Hiroki Mori and Mitsuo Shimada and Toru Ikegami and Tohru Utsunomiya and Satoru Imura and Yuji Morine and Tetsuya Ikemoto and Jun Hanaoka and Shuichi Iwahashi and Yu Saito and Michihito Asanoma and Shinichiro Yamada and Hidenori Miyake",
year = "2012",
month = "10",
doi = "10.5754/hge10401",
language = "English",
volume = "59",
pages = "2300--2304",
journal = "Acta hepato-splenologica",
issn = "0172-6390",
publisher = "H.G.E. Update Medical Publishing Ltd.",
number = "119",

}

TY - JOUR

T1 - The impact of pegylated-interferon α-2b on partial and massive hepatectomy model in rats

AU - Mori, Hiroki

AU - Shimada, Mitsuo

AU - Ikegami, Toru

AU - Utsunomiya, Tohru

AU - Imura, Satoru

AU - Morine, Yuji

AU - Ikemoto, Tetsuya

AU - Hanaoka, Jun

AU - Iwahashi, Shuichi

AU - Saito, Yu

AU - Asanoma, Michihito

AU - Yamada, Shinichiro

AU - Miyake, Hidenori

PY - 2012/10

Y1 - 2012/10

N2 - Background/Aims: The impact of pegylated-interferon (PEG-IFN) α-2b on liver regeneration has not yet been elucidated. Methodology: Rats were divided into the following four groups: 70% hepatectomy (Hx); 70% Hx+PEG-IFN; 90% Hx and 90% Hx+PEG-IFN group (n=6 each). Rats were pretreated with subcutaneous of PEG-IFN αa-2b (1.5μg/kg) administration 24 hours before Hx. Samples were taken 24, 48 and 72 hours after Hx and the following parameters were investigated: blood analysis (AST, WBC, PLT); liver weight to body weight ratio (Lw/Bw ratio); survival and PCNA labeling index (LI). Results: In the 90% Hx model, there was no significant difference between the Hx+PEG-IFN group and the Hx alone group in blood analysis; AST after postoperative 24 hours (2511 vs. 2466IU/L), WBC (1200 vs. 1290) and PLT (107 vs. 111 × 10 4/mm3), in Lw/Bw ratio at postoperative 0, 24, 48, 72 hours, respectively (0.38, 0.60, 1.14, 1.69 vs. 0.37, 0.64, 1.12, 1.63), in postoperative survival (40% vs. 45%), and in PCNA LI at postoperative 0, 24, 48, 72 hours, respectively (10.4%, 16.8%, 14.6%, 12.8% vs. 10.0%, 17.1%, 15.6%, 13.7%). In the 70% Hx model, there was no significant difference between the Hx+PEG-IFN group and the Hx alone group for all parameters. Conclusions: Our data demonstrated that PEG-IFN α-2b did not affect liver regeneration and the early use of PEG-IFN α-2b would cause no problems after liver transplantation using partial grafts including living donor liver transplantation.

AB - Background/Aims: The impact of pegylated-interferon (PEG-IFN) α-2b on liver regeneration has not yet been elucidated. Methodology: Rats were divided into the following four groups: 70% hepatectomy (Hx); 70% Hx+PEG-IFN; 90% Hx and 90% Hx+PEG-IFN group (n=6 each). Rats were pretreated with subcutaneous of PEG-IFN αa-2b (1.5μg/kg) administration 24 hours before Hx. Samples were taken 24, 48 and 72 hours after Hx and the following parameters were investigated: blood analysis (AST, WBC, PLT); liver weight to body weight ratio (Lw/Bw ratio); survival and PCNA labeling index (LI). Results: In the 90% Hx model, there was no significant difference between the Hx+PEG-IFN group and the Hx alone group in blood analysis; AST after postoperative 24 hours (2511 vs. 2466IU/L), WBC (1200 vs. 1290) and PLT (107 vs. 111 × 10 4/mm3), in Lw/Bw ratio at postoperative 0, 24, 48, 72 hours, respectively (0.38, 0.60, 1.14, 1.69 vs. 0.37, 0.64, 1.12, 1.63), in postoperative survival (40% vs. 45%), and in PCNA LI at postoperative 0, 24, 48, 72 hours, respectively (10.4%, 16.8%, 14.6%, 12.8% vs. 10.0%, 17.1%, 15.6%, 13.7%). In the 70% Hx model, there was no significant difference between the Hx+PEG-IFN group and the Hx alone group for all parameters. Conclusions: Our data demonstrated that PEG-IFN α-2b did not affect liver regeneration and the early use of PEG-IFN α-2b would cause no problems after liver transplantation using partial grafts including living donor liver transplantation.

UR - http://www.scopus.com/inward/record.url?scp=84872077354&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872077354&partnerID=8YFLogxK

U2 - 10.5754/hge10401

DO - 10.5754/hge10401

M3 - Article

C2 - 23435145

AN - SCOPUS:84872077354

VL - 59

SP - 2300

EP - 2304

JO - Acta hepato-splenologica

JF - Acta hepato-splenologica

SN - 0172-6390

IS - 119

ER -