The importance of cGMP signaling in sensory cilia for body size regulation in Caenorhabditis elegans

Manabi Fujiwara, Takahiro Hino, Ryuta Miyamoto, Hitoshi Inada, Ikue Mori, Makoto Koga, Koji Miyahara, Yasumi Ohshima, Takeshi Ishihara

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The body size of Caenorhabditis elegans is thought to be controlled by sensory inputs because many mutants with sensory cilium structure defects exhibit small body size. The EGL-4 cGMP-dependent protein kinase acts in sensory neurons to reduce body size when animals fail to perceive sensory signals. In addition to body size control, EGL-4 regulates various other behavioral and developmental pathways, including those involved in the regulation of egg laying and chemotaxis behavior. Here we have identified gcy- 12, which encodes a receptor-type guanylyl cyclase, as a gene involved in the sensory regulation of body size. Analyses with GFP fusion constructs showed that gcy-12 is expressed in several sensory neurons and localizes to sensory cilia. Genetic analyses indicated that GCY-12 acts upstream of EGL-4 in body size control but does not affect other EGL-4 functions. Our studies indicate that the function of the GCY-12 guanylyl cyclase is to provide cGMP to the EGL-4 cGMP-dependent kinase only for limited tasks including body size regulation. We also found that the PDE-2 cyclic nucleotide phosphodiesterase negatively regulates EGL-4 in controlling body size. Thus, the cGMP level is precisely controlled by GCY-12 and PDE-2 to determine body size through EGL-4, and the defects in the sensory cilium structure may disturb the balanced control of the cGMP level. The large number of guanylyl cyclases encoded in the C. elegans genome suggests that EGL-4 exerts pleiotropic effects by partnering with different guanylyl cyclases for different downstream functions.

Original languageEnglish
Pages (from-to)1497-1510
Number of pages14
JournalGenetics
Volume201
Issue number4
DOIs
Publication statusPublished - Jan 1 2015

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Cilia
Caenorhabditis elegans
Body Size
Guanylate Cyclase
Sensory Receptor Cells
Guanylate Cyclase-Coupled Receptors
Cyclic GMP-Dependent Protein Kinases
Cyclic Nucleotides
Phosphoric Diester Hydrolases
Chemotaxis
Ovum
Phosphotransferases
Genome

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

The importance of cGMP signaling in sensory cilia for body size regulation in Caenorhabditis elegans. / Fujiwara, Manabi; Hino, Takahiro; Miyamoto, Ryuta; Inada, Hitoshi; Mori, Ikue; Koga, Makoto; Miyahara, Koji; Ohshima, Yasumi; Ishihara, Takeshi.

In: Genetics, Vol. 201, No. 4, 01.01.2015, p. 1497-1510.

Research output: Contribution to journalArticle

Fujiwara, Manabi ; Hino, Takahiro ; Miyamoto, Ryuta ; Inada, Hitoshi ; Mori, Ikue ; Koga, Makoto ; Miyahara, Koji ; Ohshima, Yasumi ; Ishihara, Takeshi. / The importance of cGMP signaling in sensory cilia for body size regulation in Caenorhabditis elegans. In: Genetics. 2015 ; Vol. 201, No. 4. pp. 1497-1510.
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