The induction of chromosome condensation in tsBN2, a temperature-sensitive mutant of BHK21, inhibited by the calmodulin antagonist, W-7

Takeharu Nishimoto, Kozo Ajiro, Masato Hirata, Katsumi Yamashita, Mutsuo Sekiguchi

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Abstract

The induction of premature chromosome condensation (PCC) in tsBN2 cells, a temperature-sensitive (ts) mutant of BHK21/13 which shows PCC at the non-permissive temperature, was almost completely inhibited by 40 μM W-7, an antagonist of calmodulin. The mitotic phosphorylation of histone H1 and H3 was also inhibited by W-7, W-5, a chlorine-deficient analogue of W-7 and which interacts weakly with calmodulin, did not inhibit the induction of PCC, even at a dose of 80 μM. The content of calmodulin in tsBN2 cells was increased by a temperature shift to 40.5°C. All these results suggested that calmodulin is required for the chromosome condensation.

Original languageEnglish
Pages (from-to)351-358
Number of pages8
JournalExperimental Cell Research
Volume156
Issue number2
DOIs
Publication statusPublished - Feb 1985

All Science Journal Classification (ASJC) codes

  • Cell Biology

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