TY - JOUR
T1 - The influence of ceramide and its dihydro analog on the physico-chemical properties of sphingomyelin bilayers
AU - Kinoshita, Masanao
AU - Tanaka, Kaoru
AU - Matsumori, Nobuaki
N1 - Funding Information:
This work was supported by Japan Society for the Promotion of Science KAKENHI ( 17K15107 ) and in part JST ERATO (the Lipid Active Structure Project) and Kyushu University Interdisciplinary Programs in Education and Projects in Research Development (P&P) .
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/1
Y1 - 2020/1
N2 - The influence of ceramide and its dihydro analog (Cer and DHCer, respectively; inclusively termed Cers) on sphingomyelin (SM) bilayers was examined. Fluorescent microscopy showed that SM/Cers binary bilayers undergo phase separation between Cers-rich and Cers-poor phases. Based on calorimetry, the content of Cers in the Cers-rich phase was estimated and the results show that DHCer in the DHCer-rich phase (17.5 mol%) is more condensed than Cer in the Cer-rich phase (15 mol%), likely due to a stronger intermolecular interaction of DHCer than that of Cer. Furthermore, the Cers-poor phase consists of almost pure SM. X-ray diffraction and water permeability measurements disclosed that the size of crystallites—lipid nano-clusters formed inside the Cers-rich phase—are larger for the DHCer-rich phase than those for the Cer-rich phase. Due to its stronger intermolecular interactions, DHCer could effectively suppress the inter-crystallite packing gaps to avoid the energetic disadvantage, resulting in formation of larger crystallites.
AB - The influence of ceramide and its dihydro analog (Cer and DHCer, respectively; inclusively termed Cers) on sphingomyelin (SM) bilayers was examined. Fluorescent microscopy showed that SM/Cers binary bilayers undergo phase separation between Cers-rich and Cers-poor phases. Based on calorimetry, the content of Cers in the Cers-rich phase was estimated and the results show that DHCer in the DHCer-rich phase (17.5 mol%) is more condensed than Cer in the Cer-rich phase (15 mol%), likely due to a stronger intermolecular interaction of DHCer than that of Cer. Furthermore, the Cers-poor phase consists of almost pure SM. X-ray diffraction and water permeability measurements disclosed that the size of crystallites—lipid nano-clusters formed inside the Cers-rich phase—are larger for the DHCer-rich phase than those for the Cer-rich phase. Due to its stronger intermolecular interactions, DHCer could effectively suppress the inter-crystallite packing gaps to avoid the energetic disadvantage, resulting in formation of larger crystallites.
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U2 - 10.1016/j.chemphyslip.2019.104835
DO - 10.1016/j.chemphyslip.2019.104835
M3 - Article
C2 - 31647901
AN - SCOPUS:85074019284
VL - 226
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids
SN - 0009-3084
M1 - 104835
ER -