TY - JOUR
T1 - The Influence of Proteins and Speed on Friction and Adsorption of Metal/UHMWPE Contact Pair
AU - Nečas, D.
AU - Sawae, Y.
AU - Fujisawa, T.
AU - Nakashima, K.
AU - Morita, T.
AU - Yamaguchi, T.
AU - Vrbka, M.
AU - Křupka, I.
AU - Hartl, M.
N1 - Funding Information:
The research was supported by the project no. FSI-S-14-2336 with the financial support from the Ministry of Education, Youth and Sports of the Czech Republic. The part of this study was also supported by Grant-in-Aid for Specially Promoted Research (23000011) and Grant-in-Aid for Scientific Research (B) of Japan Society for the Promotion of Science (23360076). Authors would like to thank S. Onitsuka for supporting our FT-IR measurement.
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/9
Y1 - 2017/9
N2 - It was reported in several studies that friction and wear of joint prosthesis are apparently influenced by the proteins contained in the synovial fluid. However, detailed mechanisms of these tribological processes have not been clarified yet. The present study aims on the effect of adsorbed protein film on frictional behaviour of metal/polyethylene contact pair. Reciprocating pin-on-plate test was conducted, while the CoCrMo pin was sliding against UHMWPE plate. The contact was lubricated by various solutions of albumin and γ-globulin solved in PBS. After the friction test, the thickness of adsorbed film was evaluated using spectroscopic ellipsometry. Structure of the adsorbed proteins was later examined by FT-IR. The results showed that at low sliding speed equal to 10 mm/s, there was a linear correlation between the friction coefficient and the thickness of the adsorbed protein film. An increase of friction was thus accompanied by the evolution of protein film. In that case, both proteins undergone substantial conformational changes, losing their original structure. On the contrary, proteins could sustain their secondary structure to some extent at higher sliding speed (50 mm/s), when different behaviour of the both proteins could be observed. This phenomenon was attributed to different structure of albumin and γ-globulin in its native state. It might be concluded that friction coefficient of metal-on-polyethylene joint prosthesis is influenced by protein content, as well as kinematic conditions, since the sliding conditions have a certain effect on both the adsorbed film formation and structure of the adsorbed molecules.
AB - It was reported in several studies that friction and wear of joint prosthesis are apparently influenced by the proteins contained in the synovial fluid. However, detailed mechanisms of these tribological processes have not been clarified yet. The present study aims on the effect of adsorbed protein film on frictional behaviour of metal/polyethylene contact pair. Reciprocating pin-on-plate test was conducted, while the CoCrMo pin was sliding against UHMWPE plate. The contact was lubricated by various solutions of albumin and γ-globulin solved in PBS. After the friction test, the thickness of adsorbed film was evaluated using spectroscopic ellipsometry. Structure of the adsorbed proteins was later examined by FT-IR. The results showed that at low sliding speed equal to 10 mm/s, there was a linear correlation between the friction coefficient and the thickness of the adsorbed protein film. An increase of friction was thus accompanied by the evolution of protein film. In that case, both proteins undergone substantial conformational changes, losing their original structure. On the contrary, proteins could sustain their secondary structure to some extent at higher sliding speed (50 mm/s), when different behaviour of the both proteins could be observed. This phenomenon was attributed to different structure of albumin and γ-globulin in its native state. It might be concluded that friction coefficient of metal-on-polyethylene joint prosthesis is influenced by protein content, as well as kinematic conditions, since the sliding conditions have a certain effect on both the adsorbed film formation and structure of the adsorbed molecules.
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U2 - 10.1016/j.biotri.2017.03.003
DO - 10.1016/j.biotri.2017.03.003
M3 - Article
AN - SCOPUS:85018920034
VL - 11
SP - 51
EP - 59
JO - Biotribology
JF - Biotribology
SN - 2352-5738
ER -