The influence on the vital tissue of “GP-solvent” Newly designed olvent for the removal of guttapercha points Comparative study on the irritation effect to oral mucosa by “GP-Solvent” and chloroform

In the preceding paper, we reported that the inflammatory response of the local site induced by intradermal injection of “GP-Solvent” was significantly milder than that produced by chloroform. In the present paper the inflammatory reaction induced by “GP-Solvent” on the oral mucosa of rabbits was compared with that induced by chloroform. The results were as follows: 1) The monitoring of serum protein leakage with Evans blue showed the increased vascular permeability in the oral mucosa when chloroform was applied topically on the mucosal surface for 5 minutes, while little effect appeared even after the application of “GP-Solvent” for 20 minutes. 2) The process of inflammation in oral mucosa was observed for 14 days after “GP-Solvent” and chloroform application for 5, 10, 20, 40 and 60 minutes respectively on the mucosal surface. With “GP-Solvent” contact even for 60 minutes, we did not find any changes on the application site during 14 days. However, chloroform contact for only 5 minutes caused the severe ulceration of the application site after only one day. The above results proved that chloroform injured the oral mucosa seriously in contrast with “GP-Solvent.”.