The inhibition of basic fibroblast growth factor-induced corneal angiogenesis by D-α-tocopheryl acid succinate

T. Nakamura, Y. Oshima, T. Sakamoto, A. Matsunaga, Tatsuro Ishibashi, H. Inomata

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Abstract

Purpose. To investigate the inhibitory effect of d-α-tocopheryl acid succinate (vitamin E succinate)(VES) on angiogenesis in a rabbit corneal neovascularization model. Methods. A corneal micropocket assay was used in rabbits: 750ng of VES (n=10) or other vitamin E (vit E) analogues, vit E nicotinate, γ-tocopherol, vitamin E, or phosphate-buffered saline(PBS) (n=5 each) were randomly put in ethylenevinylacetate copolymer (EVA) pellates which were implanted in corneal micropockets in rabbits. The pockets were dissected from the center of the cornea stopping 2mm short of the limbus. Each eye was implanted with another pellet containing 200ng basic fibroblast growth factor (bFGF) central to the first pellet. Each eye was examined daily, for angiogenesis by measuring the length of new blood vessels. A histopathological analysis was also performed by electron microscopy (EM). Results. Dense neovascularization was observed in all rabits' eyes which were implanted with bFGF and PBS pellets. However the corneas implanted with VES pellets had significantly reduced neovascularization compared with corneas treated with other vit E analogues or PBS. EM study revealed less inflammation with more apoptotic cells in corneas treated VES. Conclusion. VES has an inhibitory effect on corneal angiogenesis. VES has a therapeutic potential for ocular angiogenesis.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
Publication statusPublished - Feb 15 1996

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Corneal Neovascularization
Succinic Acid
Fibroblast Growth Factor 2
Vitamin E
Acids
Cornea
Tocopherols
Phosphates
Rabbits
Electron Microscopy
Niacin
Blood Vessels
Inflammation

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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The inhibition of basic fibroblast growth factor-induced corneal angiogenesis by D-α-tocopheryl acid succinate. / Nakamura, T.; Oshima, Y.; Sakamoto, T.; Matsunaga, A.; Ishibashi, Tatsuro; Inomata, H.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

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T1 - The inhibition of basic fibroblast growth factor-induced corneal angiogenesis by D-α-tocopheryl acid succinate

AU - Nakamura, T.

AU - Oshima, Y.

AU - Sakamoto, T.

AU - Matsunaga, A.

AU - Ishibashi, Tatsuro

AU - Inomata, H.

PY - 1996/2/15

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N2 - Purpose. To investigate the inhibitory effect of d-α-tocopheryl acid succinate (vitamin E succinate)(VES) on angiogenesis in a rabbit corneal neovascularization model. Methods. A corneal micropocket assay was used in rabbits: 750ng of VES (n=10) or other vitamin E (vit E) analogues, vit E nicotinate, γ-tocopherol, vitamin E, or phosphate-buffered saline(PBS) (n=5 each) were randomly put in ethylenevinylacetate copolymer (EVA) pellates which were implanted in corneal micropockets in rabbits. The pockets were dissected from the center of the cornea stopping 2mm short of the limbus. Each eye was implanted with another pellet containing 200ng basic fibroblast growth factor (bFGF) central to the first pellet. Each eye was examined daily, for angiogenesis by measuring the length of new blood vessels. A histopathological analysis was also performed by electron microscopy (EM). Results. Dense neovascularization was observed in all rabits' eyes which were implanted with bFGF and PBS pellets. However the corneas implanted with VES pellets had significantly reduced neovascularization compared with corneas treated with other vit E analogues or PBS. EM study revealed less inflammation with more apoptotic cells in corneas treated VES. Conclusion. VES has an inhibitory effect on corneal angiogenesis. VES has a therapeutic potential for ocular angiogenesis.

AB - Purpose. To investigate the inhibitory effect of d-α-tocopheryl acid succinate (vitamin E succinate)(VES) on angiogenesis in a rabbit corneal neovascularization model. Methods. A corneal micropocket assay was used in rabbits: 750ng of VES (n=10) or other vitamin E (vit E) analogues, vit E nicotinate, γ-tocopherol, vitamin E, or phosphate-buffered saline(PBS) (n=5 each) were randomly put in ethylenevinylacetate copolymer (EVA) pellates which were implanted in corneal micropockets in rabbits. The pockets were dissected from the center of the cornea stopping 2mm short of the limbus. Each eye was implanted with another pellet containing 200ng basic fibroblast growth factor (bFGF) central to the first pellet. Each eye was examined daily, for angiogenesis by measuring the length of new blood vessels. A histopathological analysis was also performed by electron microscopy (EM). Results. Dense neovascularization was observed in all rabits' eyes which were implanted with bFGF and PBS pellets. However the corneas implanted with VES pellets had significantly reduced neovascularization compared with corneas treated with other vit E analogues or PBS. EM study revealed less inflammation with more apoptotic cells in corneas treated VES. Conclusion. VES has an inhibitory effect on corneal angiogenesis. VES has a therapeutic potential for ocular angiogenesis.

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