TY - JOUR
T1 - The inhibitory effect on neurite outgrowth of moroneurons exerted by the ligands ELF-1 and RAGS
AU - Ohta, Kunimasa
AU - Iwamasa, Hiroko
AU - Drescher, Uwe
AU - Terasaki, Hidenori
AU - Tanaka, Hideaki
N1 - Funding Information:
We thank Dr. K. Shirabe and Mrs. R. Drescher for helpful comments on the manuscript. This work was supported in part by Grants-in-aid of the Ministry of Education, Science and Culture of Japan to K.O. and H.T., and by the Science and Technology Agency, with Special Coordinating funds for Promoting Science and Technology to H.T.
PY - 1997/6
Y1 - 1997/6
N2 - Eph-related receptor tyrosine kinases and ligands are expressed at high levels in the developing nervous system, giving rise to the proposal that they are involved in neuronal connection. Cek8 was found to be predominantly expressed on a subset of motoneurons innervating limb but not body muscles during motoneuron axonal growth. Here we show that the ligands RAGS and ELF- 1 were expressed in limb buds and that they activated Cek8 when presented in membrane-bound or clustered forms of Fc chimeric proteins but not in unclustered soluble forms. When chick embryonic motoneurons enriched by panning were cultured on clustered forms of RAGS-Fc and ELF-1-Fc, the neurite growth of moroneurons expressing Cek8 was inhibited. Our results show a relationship between receptor phosphorylation and neurite growth inhibition and suggest that Eph-related kinases and ligands have a regulatory effect on the axon growth of motoneurons during development.
AB - Eph-related receptor tyrosine kinases and ligands are expressed at high levels in the developing nervous system, giving rise to the proposal that they are involved in neuronal connection. Cek8 was found to be predominantly expressed on a subset of motoneurons innervating limb but not body muscles during motoneuron axonal growth. Here we show that the ligands RAGS and ELF- 1 were expressed in limb buds and that they activated Cek8 when presented in membrane-bound or clustered forms of Fc chimeric proteins but not in unclustered soluble forms. When chick embryonic motoneurons enriched by panning were cultured on clustered forms of RAGS-Fc and ELF-1-Fc, the neurite growth of moroneurons expressing Cek8 was inhibited. Our results show a relationship between receptor phosphorylation and neurite growth inhibition and suggest that Eph-related kinases and ligands have a regulatory effect on the axon growth of motoneurons during development.
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U2 - 10.1016/S0925-4773(97)00056-7
DO - 10.1016/S0925-4773(97)00056-7
M3 - Article
C2 - 9232603
AN - SCOPUS:0030743413
VL - 64
SP - 127
EP - 135
JO - Cell Differentiation and Development
JF - Cell Differentiation and Development
SN - 0925-4773
IS - 1-2
ER -