TY - JOUR
T1 - The inhibitory effects of mushroom extracts on sucrose-dependent oral biofilm formation
AU - Yano, Akira
AU - Kikuchi, Sayaka
AU - Yamashita, Yoshihisa
AU - Sakamoto, Yuichi
AU - Nakagawa, Yuko
AU - Yoshida, Yasuo
N1 - Funding Information:
Acknowledgements This study was supported in part by Grants-in-Aid for Scientific Research numbers 21792165 and 20592181, and for High-Tech Research Projects (2009–2010 and 2005–2009, respectively) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/3
Y1 - 2010/3
N2 - Mushrooms contain large quantities of α-glucans. Shiitake (Lentinula edodes), Japan's most popular edible mushroom, has been reported to contain about 6% (weight/dried weight) of α-(1,3)-glucan. This glucan is one of the major components of oral biofilm formed by the cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. We found that extracts from shiitake and other edible mushrooms could reduce preformed biofilms of S. mutans and S. sobrinus in the presence of dextranase. We also investigated the α-glucanase activities of shiitake mushroom extracts and their effects on biofilm formation. The extracts possessed α-glucanase activity and degraded water-insoluble glucans from mutans streptococci. The extracts strongly inhibited the sucrose-dependent formation of biofilms by S. mutans and S. sobrinus in the presence of dextranase. Our results suggest that some components of mushrooms, including α-glucanases, might inhibit the sucrose-induced formation of oral biofilms.
AB - Mushrooms contain large quantities of α-glucans. Shiitake (Lentinula edodes), Japan's most popular edible mushroom, has been reported to contain about 6% (weight/dried weight) of α-(1,3)-glucan. This glucan is one of the major components of oral biofilm formed by the cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. We found that extracts from shiitake and other edible mushrooms could reduce preformed biofilms of S. mutans and S. sobrinus in the presence of dextranase. We also investigated the α-glucanase activities of shiitake mushroom extracts and their effects on biofilm formation. The extracts possessed α-glucanase activity and degraded water-insoluble glucans from mutans streptococci. The extracts strongly inhibited the sucrose-dependent formation of biofilms by S. mutans and S. sobrinus in the presence of dextranase. Our results suggest that some components of mushrooms, including α-glucanases, might inhibit the sucrose-induced formation of oral biofilms.
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U2 - 10.1007/s00253-009-2323-y
DO - 10.1007/s00253-009-2323-y
M3 - Article
C2 - 19902205
AN - SCOPUS:77249159827
VL - 86
SP - 615
EP - 623
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 2
ER -