TY - JOUR
T1 - The initiator function of DnaA protein is negatively regulated by the sliding clamp of the E. coli Chromosomal replicase
AU - Katayama, Tsutomu
AU - Kubota, Toshio
AU - Kurokawa, Kenji
AU - Crooke, Elliott
AU - Sekimizu, Kazuhisa
N1 - Funding Information:
We are grateful to Dr. Hisaji Maki, Dr. Satoko Maki, and Dr. Mike O’Donnell for providing highly purified pol III*, its subassemblies, and valuable suggestions. We are also indebted to Dr. Yoshimasa Sakakibara and Dr. Deog Su Hwang for the dnaN mutant and pBS oriC ΔLMR, respectively. Large scale culture was done by Kyowa Hakko Kogyo Co. T. Kubota was a recipient of a predoctoral fellowship from the Japan Society for the Promotion of Science. This work was in part supported by Grants-in-aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan and by grants from the National Institutes of Health (GM49700) and the National Science Foundation (MCB9408830).
PY - 1998/7/10
Y1 - 1998/7/10
N2 - The β subunit of DNA polymerase III is essential for negative regulation of the initiator protein, DnaA. DnaA inactivation occurs through accelerated hydrolysis of ATP bound to DnaA; the resulting ADP-DnaA fails to initiate replication. The ability of β subunit to promote DnaA inactivation depends on its assembly as a sliding clamp on DNA and must be accompanied by a partially purified factor, IdaB protein. DnaA inactivation in the presence of IdaB and DNA polymerase III is further stimulated by DNA synthesis, indicating close linkage between initiator inactivation and replication. In vivo, DnaA predominantly takes on the ADP form in a β subunit-dependent manner. Thus, the initiator is negatively regulated by action of the replicase, a mechanism that may be key to effective control of the replication cycle.
AB - The β subunit of DNA polymerase III is essential for negative regulation of the initiator protein, DnaA. DnaA inactivation occurs through accelerated hydrolysis of ATP bound to DnaA; the resulting ADP-DnaA fails to initiate replication. The ability of β subunit to promote DnaA inactivation depends on its assembly as a sliding clamp on DNA and must be accompanied by a partially purified factor, IdaB protein. DnaA inactivation in the presence of IdaB and DNA polymerase III is further stimulated by DNA synthesis, indicating close linkage between initiator inactivation and replication. In vivo, DnaA predominantly takes on the ADP form in a β subunit-dependent manner. Thus, the initiator is negatively regulated by action of the replicase, a mechanism that may be key to effective control of the replication cycle.
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U2 - 10.1016/S0092-8674(00)81222-2
DO - 10.1016/S0092-8674(00)81222-2
M3 - Article
C2 - 9674428
AN - SCOPUS:0032504050
VL - 94
SP - 61
EP - 71
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -