The interaction between GATA proteins and activator protein-1 promotes the transcription of IL-13 in mast cells

Akio Masuda, Yasunobu Yoshikai, Hiroaki Kume, Tetsuya Matsuguchi

    Research output: Contribution to journalArticle

    37 Citations (Scopus)

    Abstract

    IL-13 is considered to be a key modulator in the pathogenesis of Th1-induced allergic inflammation, although little is known about the regulation of IL-13 transcription in mast cells. In T cells, involvement of GATA-3 in cell type-specific expression of the IL-13 gene has been reported. However, the mechanisms that induce rapid transactivation of the IL-13 gene in response to various types of stimulation have hitherto remained unknown. In this report, we describe our investigation of the promoter region necessary for IL-13 transcription; we have found that both AP-1 and GATA proteins are indispensable for IL-13 transcription in mouse mast cells. In our investigation, we focused on the functional interaction between GATA and AP-1 in the IL-13 promoter contest Transfection experiments have revealed that GATA-1 and GATA-2 proteins are able to associate with AP-1 proteins. We have also shown that overexpression of GATA-1 induced excess AP-1 binding to the IL-13 promoter as well as a significant increase in IL-13 production in mast cells. The results of the present study have shown that direct interaction between AP-1 and GATA proteins plays an important role in IL-13 transcription in mast cells.

    Original languageEnglish
    Pages (from-to)5564-5573
    Number of pages10
    JournalJournal of Immunology
    Volume173
    Issue number9
    DOIs
    Publication statusPublished - Nov 1 2004

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

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