The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP-DnaA-specific initiation complexes

Kenji Keyamura, Norie Fujikawa, Takuma Ishida, Shogo Ozaki, Masayuki Su'etsugu, Kazuyuki Fujimitsu, Wataru Kagawa, Shigeyuki Yokoyama, Hitoshi Kurumizaka, Tsutomu Katayama

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Abstract

Escherichia coli DiaA is a DnaA-binding protein that is required for the timely initiation of chromosomal replication during the cell cycle. In this study, we determined the crystal structure of DiaA at 1.8 Å resolution. DiaA forms a homotetramer consisting of a symmetrical pair of homodimers. Mutational analysis revealed that the DnaA-binding activity and formation of homotetramers are required for the stimulation of initiation by DiaA. DiaA tetramers can bind multiple DnaA molecules simultaneously. DiaA stimulated the assembly of multiple DnaA molecules on oriC, conformational changes in ATP-DnaA-specific initiation complexes, and unwinding of oriC duplex DNA. The mutant DiaA proteins are defective in these stimulations. DiaA associated also with ADP-DnaA, and stimulated the assembly of inactive ADP-DnaA-oriC complexes. Specific residues in the putative phosphosugar-binding motif of DiaA were required for the stimulation of initiation and formation of ATP-DnaA-specific- oriC complexes. Our data indicate that DiaA regulates initiation by a novel mechanism, in which DiaA tetramers most likely bind to multiple DnaA molecules and stimulate the assembly of specific ATP-DnaA-oriC complexes. These results suggest an essential role for DiaA in the promotion of replication initiation in a cell cycle coordinated manner.

Original languageEnglish
Pages (from-to)2083-2099
Number of pages17
JournalGenes and Development
Volume21
Issue number16
DOIs
Publication statusPublished - Aug 15 2007

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Adenosine Triphosphate
Escherichia coli
Adenosine Diphosphate
Cell Cycle
Mutant Proteins
Carrier Proteins
DNA

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP-DnaA-specific initiation complexes. / Keyamura, Kenji; Fujikawa, Norie; Ishida, Takuma; Ozaki, Shogo; Su'etsugu, Masayuki; Fujimitsu, Kazuyuki; Kagawa, Wataru; Yokoyama, Shigeyuki; Kurumizaka, Hitoshi; Katayama, Tsutomu.

In: Genes and Development, Vol. 21, No. 16, 15.08.2007, p. 2083-2099.

Research output: Contribution to journalArticle

Keyamura, K, Fujikawa, N, Ishida, T, Ozaki, S, Su'etsugu, M, Fujimitsu, K, Kagawa, W, Yokoyama, S, Kurumizaka, H & Katayama, T 2007, 'The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP-DnaA-specific initiation complexes', Genes and Development, vol. 21, no. 16, pp. 2083-2099. https://doi.org/10.1101/gad.1561207
Keyamura, Kenji ; Fujikawa, Norie ; Ishida, Takuma ; Ozaki, Shogo ; Su'etsugu, Masayuki ; Fujimitsu, Kazuyuki ; Kagawa, Wataru ; Yokoyama, Shigeyuki ; Kurumizaka, Hitoshi ; Katayama, Tsutomu. / The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP-DnaA-specific initiation complexes. In: Genes and Development. 2007 ; Vol. 21, No. 16. pp. 2083-2099.
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