The interrelationship between abscisic acid and reactive oxygen species plays a key role in barley seed dormancy and germination

Yushi Ishibashi, Nozomi Aoki, Shinsuke Kasa, Masatsugu Sakamoto, Kyohei Kai, Reisa Tomokiyo, Gaku Watabe, Takashi Yuasa, Mari Iwaya-Inoue

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Seed dormancy is one of the adaptive responses in the plant life cycle and an important agronomic trait. Reactive oxygen species (ROS) release seed dormancy and promote seed germination in several cereal crops; however, the key regulatory mechanism of ROS-mediated seed dormancy and germination remains controversial. Here, we focused on the relationship between hydrogen peroxide (a ROS) and abscisic acid (ABA) in dormant and non-dormant barley seeds. The hydrogen peroxide (H2O2) level produced in barley seed embryos after imbibition was higher in non-dormant seeds than in dormant seeds. H2O2regulated the ABA content in the embryos through ABA-80- hydroxylase, an ABA catabolic enzyme. Moreover, compared with non-dormant seeds, in dormant seeds the activity of NADPH oxidase, which produces ROS, was lower, whereas the activity of catalase, which is a H2O2scavenging enzyme, was higher, as was the expression of HvCAT2. Furthermore, precocious germination of isolated immature embryos was suppressed by the transient introduction of HvCAT2 driven by the maize (Zea mays) ubiquitin promoter. HvCAT2 expression was regulated through an ABA-responsive transcription factor (HvABI5) induced by ABA. These results suggest that the changing of balance between ABA and ROS is active in barley seed embryos after imbibition and regulates barley seed dormancy and germination.

Original languageEnglish
Article number275
JournalFrontiers in Plant Science
Volume8
DOIs
Publication statusPublished - Mar 21 2017

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seed dormancy
abscisic acid
reactive oxygen species
seed germination
barley
embryo (plant)
seeds
imbibition
hydrogen peroxide
ubiquitin
enzymes
grain crops
agronomic traits
plant response
life cycle (organisms)
catalase
transcription factors
Zea mays
immatures
promoter regions

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

The interrelationship between abscisic acid and reactive oxygen species plays a key role in barley seed dormancy and germination. / Ishibashi, Yushi; Aoki, Nozomi; Kasa, Shinsuke; Sakamoto, Masatsugu; Kai, Kyohei; Tomokiyo, Reisa; Watabe, Gaku; Yuasa, Takashi; Iwaya-Inoue, Mari.

In: Frontiers in Plant Science, Vol. 8, 275, 21.03.2017.

Research output: Contribution to journalArticle

Ishibashi, Yushi ; Aoki, Nozomi ; Kasa, Shinsuke ; Sakamoto, Masatsugu ; Kai, Kyohei ; Tomokiyo, Reisa ; Watabe, Gaku ; Yuasa, Takashi ; Iwaya-Inoue, Mari. / The interrelationship between abscisic acid and reactive oxygen species plays a key role in barley seed dormancy and germination. In: Frontiers in Plant Science. 2017 ; Vol. 8.
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