The involvement of the 67 kDa laminin receptor-mediated modulation of cytoskeleton in the degranulation inhibition induced by epigallocatechin-3-O-gallate

Yoshinori Fujimura, Daisuke Umeda, Yuko Kiyohara, Yousuke Sunada, Koji Yamada, Hirofumi Tachibana

Research output: Contribution to journalArticle

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Abstract

Recently, we have reported that (-)-epigallocatechin-3-O-gallate (EGCG) acts as an inhibitor of degranulation. However, the inhibitory mechanism for degranulation is still poorly understood. Here we show that suppression of exocytosis-related myosin II regulatory light chain phosphorylation and alteration of actin remodeling are involved in the inhibitory effect of EGCG on the calcium ionophore-induced degranulation from human basophilic KU812 cells. Surface plasmon resonance assay also revealed that EGCG binds to the cell surface, and the disruption of lipid rafts resulted in reduction of EGCG's ability. We have previously identified the raft-associated 67 kDa laminin receptor (67LR) as an EGCG receptor on the cell surface. Treatment of the cells with anti-67LR antibody or RNA interference-mediated downregulation of 67LR expression abolished the effects of EGCG. These findings suggest that EGCG-induced inhibition of the degranulation includes the primary binding of EGCG to the cell surface 67LR and subsequent modulation of cytoskeleton.

Original languageEnglish
Pages (from-to)524-531
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume348
Issue number2
DOIs
Publication statusPublished - Sep 22 2006

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Laminin Receptors
Cytoskeleton
Modulation
Myosin Type II
Myosin Light Chains
Phosphorylation
Surface Plasmon Resonance
Calcium Ionophores
Exocytosis
Cell Surface Receptors
Surface plasmon resonance
RNA Interference
epigallocatechin gallate
Actins
Assays
Down-Regulation
Cells
RNA
Lipids
Antibodies

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The involvement of the 67 kDa laminin receptor-mediated modulation of cytoskeleton in the degranulation inhibition induced by epigallocatechin-3-O-gallate. / Fujimura, Yoshinori; Umeda, Daisuke; Kiyohara, Yuko; Sunada, Yousuke; Yamada, Koji; Tachibana, Hirofumi.

In: Biochemical and Biophysical Research Communications, Vol. 348, No. 2, 22.09.2006, p. 524-531.

Research output: Contribution to journalArticle

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