The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism

Marcus Braun, Douglas Robert Drummond, Robert A. Cross, Andrew D. McAinsh

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

The dynamic organization of microtubules into parallel arrays allows interphase cells to set up multi-lane highways for intracellular transport and M-phase cells to build the mitotic and meiotic spindles. Here we show that a minimally reconstituted system composed of Klp2, a kinesin-14 from the fission yeast Schizosaccharomyces pombe, together with microtubules assembled from purified S. pombe tubulin, autonomously assembles bundles of parallel microtubules. Bundles form by an ATP-dependent sorting mechanism that requires the full-length Klp2 motor. By this mechanism, antiparallel-overlapped microtubules slide over one another until they dissociate from the bundles, whereas parallel-overlapped microtubules are selectively trapped by an energy-dissipating force-balance mechanism. Klp2-driven microtubule sorting provides a robust pathway for the organization of microtubules into parallel arrays. In vivo evidence indicates that Klp2 is required for the proper organization of S. pombe interphase microtubules into bipolar arrays of parallel-overlapped microtubules, suggesting that kinesin-14-dependent microtubule sorting may have wide biological importance.

Original languageEnglish
Pages (from-to)724-730
Number of pages7
JournalNature Cell Biology
Volume11
Issue number6
DOIs
Publication statusPublished - May 11 2009
Externally publishedYes

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Kinesin
Microtubules
Adenosine Triphosphate
Schizosaccharomyces
Spindle Apparatus
Interphase
Tubulin
Cell Division

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism. / Braun, Marcus; Drummond, Douglas Robert; Cross, Robert A.; McAinsh, Andrew D.

In: Nature Cell Biology, Vol. 11, No. 6, 11.05.2009, p. 724-730.

Research output: Contribution to journalArticle

Braun, Marcus ; Drummond, Douglas Robert ; Cross, Robert A. ; McAinsh, Andrew D. / The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism. In: Nature Cell Biology. 2009 ; Vol. 11, No. 6. pp. 724-730.
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