The large scale production of Cerrena unicolor laccase on waste agricultural based media

Iwona Mazur, Beata Rola, Krzysztof Stolarczyk, Ewa Nazaruk, Renata Bilewicz, Jerzy Rogalski, Shoji Ohga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effect of different agriculture waste polysaccharides as an only carbon source in culture medium of wood-degrading basidiomycete Cerrena unicolor C-139 were investigated. The maximal growth and laccase synthesis in shaken flask was observed in mineral salts broth containing potato starch as the carbon source and asparagine as the nitrogen source (11,000 nkat/L). When an optimized medium was stimulated by addition of 10 μM Cu2+ to the culture medium on days 3-5 the maximal activity 24,000 nkt/L was obtained. Next the influence of the stabilization of the medium pH after 48-h incubation on lacease activity in fermenter cultures had to be estimated. The obtained data show that use of an automatic pH control set at pH 5.0 increased lacease productivity significantly (by 12 times) as compared to that obtained in the fermentar culture with a non-stabilized pH-value. Under the new conditions, the highest enzyme activity of 290,000 nkat/L was reached after 13-day incubation.

Original languageEnglish
Pages (from-to)297-302
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume60
Issue number2
Publication statusPublished - Sep 1 2015

Fingerprint

Laccase
agricultural wastes
laccase
Culture Media
Carbon
culture media
Basidiomycota
carbon
fermenters
Asparagine
potato starch
asparagine
Solanum tuberosum
Agriculture
Starch
Minerals
Polysaccharides
Nitrogen
polysaccharides
Salts

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Mazur, I., Rola, B., Stolarczyk, K., Nazaruk, E., Bilewicz, R., Rogalski, J., & Ohga, S. (2015). The large scale production of Cerrena unicolor laccase on waste agricultural based media. Journal of the Faculty of Agriculture, Kyushu University, 60(2), 297-302.

The large scale production of Cerrena unicolor laccase on waste agricultural based media. / Mazur, Iwona; Rola, Beata; Stolarczyk, Krzysztof; Nazaruk, Ewa; Bilewicz, Renata; Rogalski, Jerzy; Ohga, Shoji.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 60, No. 2, 01.09.2015, p. 297-302.

Research output: Contribution to journalArticle

Mazur, I, Rola, B, Stolarczyk, K, Nazaruk, E, Bilewicz, R, Rogalski, J & Ohga, S 2015, 'The large scale production of Cerrena unicolor laccase on waste agricultural based media', Journal of the Faculty of Agriculture, Kyushu University, vol. 60, no. 2, pp. 297-302.
Mazur I, Rola B, Stolarczyk K, Nazaruk E, Bilewicz R, Rogalski J et al. The large scale production of Cerrena unicolor laccase on waste agricultural based media. Journal of the Faculty of Agriculture, Kyushu University. 2015 Sep 1;60(2):297-302.
Mazur, Iwona ; Rola, Beata ; Stolarczyk, Krzysztof ; Nazaruk, Ewa ; Bilewicz, Renata ; Rogalski, Jerzy ; Ohga, Shoji. / The large scale production of Cerrena unicolor laccase on waste agricultural based media. In: Journal of the Faculty of Agriculture, Kyushu University. 2015 ; Vol. 60, No. 2. pp. 297-302.
@article{2c17486906a44658a75c971b2744d01a,
title = "The large scale production of Cerrena unicolor laccase on waste agricultural based media",
abstract = "The effect of different agriculture waste polysaccharides as an only carbon source in culture medium of wood-degrading basidiomycete Cerrena unicolor C-139 were investigated. The maximal growth and laccase synthesis in shaken flask was observed in mineral salts broth containing potato starch as the carbon source and asparagine as the nitrogen source (11,000 nkat/L). When an optimized medium was stimulated by addition of 10 μM Cu2+ to the culture medium on days 3-5 the maximal activity 24,000 nkt/L was obtained. Next the influence of the stabilization of the medium pH after 48-h incubation on lacease activity in fermenter cultures had to be estimated. The obtained data show that use of an automatic pH control set at pH 5.0 increased lacease productivity significantly (by 12 times) as compared to that obtained in the fermentar culture with a non-stabilized pH-value. Under the new conditions, the highest enzyme activity of 290,000 nkat/L was reached after 13-day incubation.",
author = "Iwona Mazur and Beata Rola and Krzysztof Stolarczyk and Ewa Nazaruk and Renata Bilewicz and Jerzy Rogalski and Shoji Ohga",
year = "2015",
month = "9",
day = "1",
language = "English",
volume = "60",
pages = "297--302",
journal = "Journal of the Faculty of Agriculture, Kyushu University",
issn = "0023-6152",
publisher = "Faculty of Agriculture, Kyushu University",
number = "2",

}

TY - JOUR

T1 - The large scale production of Cerrena unicolor laccase on waste agricultural based media

AU - Mazur, Iwona

AU - Rola, Beata

AU - Stolarczyk, Krzysztof

AU - Nazaruk, Ewa

AU - Bilewicz, Renata

AU - Rogalski, Jerzy

AU - Ohga, Shoji

PY - 2015/9/1

Y1 - 2015/9/1

N2 - The effect of different agriculture waste polysaccharides as an only carbon source in culture medium of wood-degrading basidiomycete Cerrena unicolor C-139 were investigated. The maximal growth and laccase synthesis in shaken flask was observed in mineral salts broth containing potato starch as the carbon source and asparagine as the nitrogen source (11,000 nkat/L). When an optimized medium was stimulated by addition of 10 μM Cu2+ to the culture medium on days 3-5 the maximal activity 24,000 nkt/L was obtained. Next the influence of the stabilization of the medium pH after 48-h incubation on lacease activity in fermenter cultures had to be estimated. The obtained data show that use of an automatic pH control set at pH 5.0 increased lacease productivity significantly (by 12 times) as compared to that obtained in the fermentar culture with a non-stabilized pH-value. Under the new conditions, the highest enzyme activity of 290,000 nkat/L was reached after 13-day incubation.

AB - The effect of different agriculture waste polysaccharides as an only carbon source in culture medium of wood-degrading basidiomycete Cerrena unicolor C-139 were investigated. The maximal growth and laccase synthesis in shaken flask was observed in mineral salts broth containing potato starch as the carbon source and asparagine as the nitrogen source (11,000 nkat/L). When an optimized medium was stimulated by addition of 10 μM Cu2+ to the culture medium on days 3-5 the maximal activity 24,000 nkt/L was obtained. Next the influence of the stabilization of the medium pH after 48-h incubation on lacease activity in fermenter cultures had to be estimated. The obtained data show that use of an automatic pH control set at pH 5.0 increased lacease productivity significantly (by 12 times) as compared to that obtained in the fermentar culture with a non-stabilized pH-value. Under the new conditions, the highest enzyme activity of 290,000 nkat/L was reached after 13-day incubation.

UR - http://www.scopus.com/inward/record.url?scp=84978040290&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978040290&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84978040290

VL - 60

SP - 297

EP - 302

JO - Journal of the Faculty of Agriculture, Kyushu University

JF - Journal of the Faculty of Agriculture, Kyushu University

SN - 0023-6152

IS - 2

ER -