TY - JOUR
T1 - The lncRNA Neat1 is required for corpus luteum formation and the establishment of pregnancy in a subpopulation of mice
AU - Nakagawa, Shinichi
AU - Shimada, Masayuki
AU - Yanaka, Kaori
AU - Mito, Mari
AU - Arai, Takashi
AU - Takahashi, Eiki
AU - Fujita, Youko
AU - Fujimori, Toshihiko
AU - Standaert, Laura
AU - Marine, Jean Christophe
AU - Hirose, Tetsuro
N1 - Publisher Copyright:
© 2014. Published by The Company of Biologists Ltd.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Neat1 is a non-protein-coding RNA that serves as an architectural component of the nuclear bodies known as paraspeckles. Although cell-based studies indicate that Neat1 is a crucial regulator of gene expression, its physiological relevance remains unclear. Here, we find that Neat1 knockout (KO) mice stochastically fail to become pregnant despite normal ovulation. Unilateral transplantation of wild-type ovaries or the administration of progesterone partially rescued the phenotype, suggesting that corpus luteum dysfunction and concomitant low progesterone were the primary causes of the decreased fertility. In contrast to the faint expression observed in most of the adult tissues, Neat1 was highly expressed in the corpus luteum, and the formation of luteal tissue was severely impaired in nearly half of the Neat1 KO mice. These observations suggest that Neat1 is essential for the formation of the corpus luteum and for the subsequent establishment of pregnancy under a suboptimal condition that has not yet been identified.
AB - Neat1 is a non-protein-coding RNA that serves as an architectural component of the nuclear bodies known as paraspeckles. Although cell-based studies indicate that Neat1 is a crucial regulator of gene expression, its physiological relevance remains unclear. Here, we find that Neat1 knockout (KO) mice stochastically fail to become pregnant despite normal ovulation. Unilateral transplantation of wild-type ovaries or the administration of progesterone partially rescued the phenotype, suggesting that corpus luteum dysfunction and concomitant low progesterone were the primary causes of the decreased fertility. In contrast to the faint expression observed in most of the adult tissues, Neat1 was highly expressed in the corpus luteum, and the formation of luteal tissue was severely impaired in nearly half of the Neat1 KO mice. These observations suggest that Neat1 is essential for the formation of the corpus luteum and for the subsequent establishment of pregnancy under a suboptimal condition that has not yet been identified.
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U2 - 10.1242/dev.110544
DO - 10.1242/dev.110544
M3 - Article
C2 - 25359727
AN - SCOPUS:84911466128
VL - 141
SP - 4618
EP - 4627
JO - Journal of Embryology and Experimental Morphology
JF - Journal of Embryology and Experimental Morphology
SN - 0950-1991
IS - 23
ER -