The localization and phosphorylation of p47 are important for Golgi disassembly-assembly during the cell cycle

Keiji Uchiyama, Eija Jokitalo, Mervi Lindman, Mark Jackman, Fumi Kano, Masayuki Murata, Xiaodong Zhang, Hisao Kondo

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Abstract

In mammalian cells, the Golgi apparatus is disassembled at the onset of mitosis and reassembled at the end of mitosis. This disassembly-reassembly is generally believed to be essential for the equal partitioning of Golgi into two daughter cells. For Golgi disassembly, membrane fusion, which is mediated by NSF and p97, needs to be blocked. For the NSF pathway, the tethering of pl 15-GM130 is disrupted by the mitotic phosphorylation of GM130, resulting in the inhibition of NSF-mediated fusion. In contrast, the p97/p47 pathway does not require p115-GM130 tethering, and its mitotic inhibitory mechanism has been unclear. Now, we have found that p47, which mainly localizes to the nucleus during interphase, is phosphorylated on Serine-140 by Cdc2 at mitosis. The phosphorylated p47 does not bind to Golgi membranes. An in vitro assay shows that this phosphorylation is required for Golgi disassembly. Microinjection of p47(S140A), which is unable to be phosphorylated, allows the cell to keep Golgi stacks during mitosis and has no effect on the equal partitioning of Golgi into two daughter cells, suggesting that Golgi fragmentation-dispersion may not be obligatory for equal partitioning even in mammalian cells.

Original languageEnglish
Pages (from-to)1067-1079
Number of pages13
JournalJournal of Cell Biology
Volume161
Issue number6
DOIs
Publication statusPublished - Jun 23 2003

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Cell Cycle
Mitosis
Phosphorylation
Membrane Fusion
Interphase
Microinjections
Golgi Apparatus
Serine
Membranes

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

The localization and phosphorylation of p47 are important for Golgi disassembly-assembly during the cell cycle. / Uchiyama, Keiji; Jokitalo, Eija; Lindman, Mervi; Jackman, Mark; Kano, Fumi; Murata, Masayuki; Zhang, Xiaodong; Kondo, Hisao.

In: Journal of Cell Biology, Vol. 161, No. 6, 23.06.2003, p. 1067-1079.

Research output: Contribution to journalArticle

Uchiyama, K, Jokitalo, E, Lindman, M, Jackman, M, Kano, F, Murata, M, Zhang, X & Kondo, H 2003, 'The localization and phosphorylation of p47 are important for Golgi disassembly-assembly during the cell cycle', Journal of Cell Biology, vol. 161, no. 6, pp. 1067-1079. https://doi.org/10.1083/jcb.200303048
Uchiyama, Keiji ; Jokitalo, Eija ; Lindman, Mervi ; Jackman, Mark ; Kano, Fumi ; Murata, Masayuki ; Zhang, Xiaodong ; Kondo, Hisao. / The localization and phosphorylation of p47 are important for Golgi disassembly-assembly during the cell cycle. In: Journal of Cell Biology. 2003 ; Vol. 161, No. 6. pp. 1067-1079.
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