The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking

Shigemi Sasawatari, Mariko Yoshizaki, Choji Taya, Aya Tazawa, Kaori Furuyama-Tanaka, Hiromichi Yonekawa, Taeko Dohi, Andrew P. Makrigiannis, Takehiko Sasazuki, Kayo Inaba, Noriko Toyama-Sorimachi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Neutrophils rapidly undergo polarization and directional movement to infiltrate the sites of infection and inflammation. Here, we show that an inhibitory MHC I receptor, Ly49Q, was crucial for the swift polarization of and tissue infiltration by neutrophils. During the steady state, Ly49Q inhibited neutrophil adhesion by preventing focal-complex formation, likely by inhibiting Src and PI3 kinases. However, in the presence of inflammatory stimuli, Ly49Q mediated rapid neutrophil polarization and tissue infiltration in an ITIM-domain-dependent manner. These opposite functions appeared to be mediated by distinct use of effector phosphatase SHP-1 and SHP-2. Ly49Q-dependent polarization and migration were affected by Ly49Q regulation of membrane raft functions. We propose that Ly49Q is pivotal in switching neutrophils to their polarized morphology and rapid migration upon inflammation, through its spatiotemporal regulation of membrane rafts and raft-associated signaling molecules.

Original languageEnglish
Pages (from-to)200-213
Number of pages14
JournalImmunity
Volume32
Issue number2
DOIs
Publication statusPublished - Feb 26 2010

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Neutrophils
Non-Receptor Type 6 Protein Tyrosine Phosphatase
Inflammation
Focal Adhesions
Membranes
src-Family Kinases
Neutrophil Infiltration
Phosphatidylinositol 3-Kinases
Infection

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Sasawatari, S., Yoshizaki, M., Taya, C., Tazawa, A., Furuyama-Tanaka, K., Yonekawa, H., ... Toyama-Sorimachi, N. (2010). The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking. Immunity, 32(2), 200-213. https://doi.org/10.1016/j.immuni.2010.01.012

The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking. / Sasawatari, Shigemi; Yoshizaki, Mariko; Taya, Choji; Tazawa, Aya; Furuyama-Tanaka, Kaori; Yonekawa, Hiromichi; Dohi, Taeko; Makrigiannis, Andrew P.; Sasazuki, Takehiko; Inaba, Kayo; Toyama-Sorimachi, Noriko.

In: Immunity, Vol. 32, No. 2, 26.02.2010, p. 200-213.

Research output: Contribution to journalArticle

Sasawatari, S, Yoshizaki, M, Taya, C, Tazawa, A, Furuyama-Tanaka, K, Yonekawa, H, Dohi, T, Makrigiannis, AP, Sasazuki, T, Inaba, K & Toyama-Sorimachi, N 2010, 'The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking', Immunity, vol. 32, no. 2, pp. 200-213. https://doi.org/10.1016/j.immuni.2010.01.012
Sasawatari S, Yoshizaki M, Taya C, Tazawa A, Furuyama-Tanaka K, Yonekawa H et al. The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking. Immunity. 2010 Feb 26;32(2):200-213. https://doi.org/10.1016/j.immuni.2010.01.012
Sasawatari, Shigemi ; Yoshizaki, Mariko ; Taya, Choji ; Tazawa, Aya ; Furuyama-Tanaka, Kaori ; Yonekawa, Hiromichi ; Dohi, Taeko ; Makrigiannis, Andrew P. ; Sasazuki, Takehiko ; Inaba, Kayo ; Toyama-Sorimachi, Noriko. / The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking. In: Immunity. 2010 ; Vol. 32, No. 2. pp. 200-213.
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