The maintenance of the endoplasmic reticulum network is regulated by p47, a cofactor of p97, through phosphorylation by cdc2 kinase

Fumi Kano, Hisao Kondo, Akitsugu Yamamoto, Arowu R. Tanaka, Nobuko Hosokawa, Kazuhiro Nagata, Masayuki Murata

Research output: Contribution to journalArticle

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Abstract

The endoplasmic reticulum (ER) has a characteristic complex polygonal structure with hallmark three-way junctions in many types of cells. To investigate the mechanisms responsible for maintaining the ER network, we established ER disassembly and reassembly assays in semi-intact Chinese hamster ovary (CHO) cells that constitutively expressed heat shock protein-47 fused to the green fluorescent protein (GFP-HSP47) as an ER marker (the cells are referred to as CHO-HSP cells). Using these assays, we found that maintenance of the ER network required cytosol and adenosine triphosphate/guanosine 5′-triphosphate (ATP/GTP) hydrolysis, but not actin filaments or microtubules. We also showed that the ER network was disrupted upon addition of either N-ethylmaleimide-treated cytosol after washing semi-intact cells with high salt solution or mitotic cytosol in nocodazole-treated semi-intact CHO-HSP cells. The disrupted ER network induced by mitotic cytosol was reformed by the addition of interphase cytosol. In addition, we found that p47, a cofactor of p97, was essential for the maintenance of the ER network, and that phosphorylation of p47 by cdc2 kinase resulted in ER network disruption by mitotic cytosol. Taken together, these results imply that the maintenance of the ER network requires a membrane fusion process mediated by p97/p47, and that cell cycle-dependent morphological changes of the ER network are regulated through phosphorylation/dephosphorylation of p47.

Original languageEnglish
Pages (from-to)333-344
Number of pages12
JournalGenes to Cells
Volume10
Issue number4
DOIs
Publication statusPublished - Apr 1 2005

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Endoplasmic Reticulum
Phosphotransferases
Maintenance
Phosphorylation
Cytosol
Cricetulus
Ovary
HSP47 Heat-Shock Proteins
rat Nsfl1c protein
Nocodazole
Membrane Fusion
Ethylmaleimide
Interphase
Guanosine Triphosphate
Green Fluorescent Proteins
Actin Cytoskeleton
Microtubules
Cell Cycle
Hydrolysis
Salts

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

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The maintenance of the endoplasmic reticulum network is regulated by p47, a cofactor of p97, through phosphorylation by cdc2 kinase. / Kano, Fumi; Kondo, Hisao; Yamamoto, Akitsugu; Tanaka, Arowu R.; Hosokawa, Nobuko; Nagata, Kazuhiro; Murata, Masayuki.

In: Genes to Cells, Vol. 10, No. 4, 01.04.2005, p. 333-344.

Research output: Contribution to journalArticle

Kano, Fumi ; Kondo, Hisao ; Yamamoto, Akitsugu ; Tanaka, Arowu R. ; Hosokawa, Nobuko ; Nagata, Kazuhiro ; Murata, Masayuki. / The maintenance of the endoplasmic reticulum network is regulated by p47, a cofactor of p97, through phosphorylation by cdc2 kinase. In: Genes to Cells. 2005 ; Vol. 10, No. 4. pp. 333-344.
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