TY - JOUR
T1 - The majority of cDNA clones with strong positive signals for the interferon-induction-specific sequences resemble mitochondrial ribosomal RNA genes
AU - Tsuzuki, Teruhisa
AU - Nomiyama, Hisayuki
AU - Setoyama, Chiaki
AU - Maeda, Shuichiro
AU - Shimada, Kazunori
AU - Pestka, Sidney
N1 - Funding Information:
DNA Isolation. Human genomic DNAs were prepared from human placenta as described by Blin and Stafford (2). Plasmid DNAs were prepared as described by Mukai et a7. (3). Propagation of A coli cells carrying recombinant plasmids was carried out in accordance with the guidelines for recombinant DNA research, issued by the Ministry of Education, Science and Culture of Japan as well as in accordance with the NIH Guidelines Recombinant DNA Research.
Funding Information:
ACKNOWLEDGMENTS: and Gynecology, Ohara for reading tific grant from We thank Drs. M. Nakayama and M. Maeyama, Dept. of Obstetrics Kumamoto University, for provision of the human placenta and M. the manuscript. This work was supported in part by a scien-the Ministry of Education, Science and Culture, Japan.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1983/7/29
Y1 - 1983/7/29
N2 - A complementary DNA library prepared from the 12S polyadenylated RNAs extracted from interferon-induced KG-1 cells, a human myeloblast cell line, was screened for the presence of induction-specific sequences. Clones that exhibited strong positive signals were separated by hybridization criteria into nine classes. Clones from classes I through IV consisted of about 78% of the total and unexpectedly were found to resemble human mitochondrial ribosomal RNA genes.
AB - A complementary DNA library prepared from the 12S polyadenylated RNAs extracted from interferon-induced KG-1 cells, a human myeloblast cell line, was screened for the presence of induction-specific sequences. Clones that exhibited strong positive signals were separated by hybridization criteria into nine classes. Clones from classes I through IV consisted of about 78% of the total and unexpectedly were found to resemble human mitochondrial ribosomal RNA genes.
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U2 - 10.1016/0006-291X(83)90833-1
DO - 10.1016/0006-291X(83)90833-1
M3 - Article
C2 - 6192820
AN - SCOPUS:0021114937
VL - 114
SP - 670
EP - 676
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -