The making of symbiont capsule in the plataspid stinkbug Megacopta punctatissima

Takahiro Hosokawa, Yoshitomo Kikuchi, Ying Meng Xien, Takema Fukatsu

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

In stinkbugs of the family Plataspidae, adult females deposit small brownish particles, containing specific symbiotic bacteria inside, on the underside of their egg mass. Newborn nymphs ingest the content of the unique structure, called "symbiont capsule", whereby vertical transmission of the symbiont occurs. We investigated the fine structure and the formation process of the symbiont capsule in the Japanese common plataspid stinkbug, Megacopta punctatissima, by using light and electron microscopy. It was demonstrated that (i) the capsule consists of three structural components, namely "symbionts", "matrix" and "envelope"; (ii) the posterior midgut of adult females is characterized by several specific sections with peculiar anatomical traits, including "thin crypt-bearing midgut (TCM) section", "swollen crypt-bearing midgut (SCM) section" and "brownish enlarged midgut (BEM) end section"; (iii) the different capsule components, symbionts, matrix and envelope, are produced and/or supplied by the specialized midgut sections, TCM, SCM and BEM, respectively; and (iv) the capsule components are stored in BEM and excreted during oviposition to produce the symbiont capsules. These results strongly suggested that the host insect incurs a substantial cost for the symbiont transmission. Ecological and evolutionary implications of the highly developed, female-specific system for symbiont transmission were discussed.

Original languageEnglish
Pages (from-to)471-477
Number of pages7
JournalFEMS microbiology ecology
Volume54
Issue number3
DOIs
Publication statusPublished - Nov 1 2005
Externally publishedYes

Fingerprint

symbiont
Capsules
Nymph
Oviposition
vertical transmission
matrix
Ovum
structural component
Insects
Electron Microscopy
electron microscopy
thin section
oviposition
Bacteria
Light
Costs and Cost Analysis
microscopy
insect
egg
bacterium

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

Cite this

The making of symbiont capsule in the plataspid stinkbug Megacopta punctatissima. / Hosokawa, Takahiro; Kikuchi, Yoshitomo; Xien, Ying Meng; Fukatsu, Takema.

In: FEMS microbiology ecology, Vol. 54, No. 3, 01.11.2005, p. 471-477.

Research output: Contribution to journalArticle

Hosokawa, Takahiro ; Kikuchi, Yoshitomo ; Xien, Ying Meng ; Fukatsu, Takema. / The making of symbiont capsule in the plataspid stinkbug Megacopta punctatissima. In: FEMS microbiology ecology. 2005 ; Vol. 54, No. 3. pp. 471-477.
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