The manner in which DNA is packaged with TFAM has an impact on transcription activation and inhibition

Ryo Furukawa; Yuma Yamada; Yuichi Matsushima; Yu Ichi Goto; Hideyoshi Harashima

doi:10.1016/j.fob.2012.06.001

The manner in which DNA is packaged with TFAM has an impact on transcription activation and inhibition

Ryo Furukawa, Yuma Yamada, Yuichi Matsushima, Yu Ichi Goto, Hideyoshi Harashima

Research output: Contribution to journal › Article

6 Citations (Scopus)

Abstract

For successful mitochondrial transgene expression, an optimal packaging exogenous DNA is an important issue. We report herein on the effects of DNA packaged with mitochondrial transcription factor A (TFAM), which packages mitochondrial DNA (mtDNA), on the transcription process. Our initial findings indicated that the transcription of the TFAM/DNA complex was activated, when the complex was formed at an optimal ratio. We also found that TFAM has a significant advantage over protamine, a nuclear DNA packaging protein, from the viewpoint of transcription efficiency. This result indicates that TFAM can be useful packaging protein for exogenous DNA to achieve mitochondrial transgene expression.

Original language	English
Pages (from-to)	145-150
Number of pages	6
Journal	FEBS Open Bio
Volume	2
DOIs	https://doi.org/10.1016/j.fob.2012.06.001
Publication status	Published - Jul 3 2012
Externally published	Yes

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All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

Cite this

Furukawa, R., Yamada, Y., Matsushima, Y., Goto, Y. I., & Harashima, H. (2012). The manner in which DNA is packaged with TFAM has an impact on transcription activation and inhibition. FEBS Open Bio, 2, 145-150. https://doi.org/10.1016/j.fob.2012.06.001

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AU - Harashima, Hideyoshi

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AB - For successful mitochondrial transgene expression, an optimal packaging exogenous DNA is an important issue. We report herein on the effects of DNA packaged with mitochondrial transcription factor A (TFAM), which packages mitochondrial DNA (mtDNA), on the transcription process. Our initial findings indicated that the transcription of the TFAM/DNA complex was activated, when the complex was formed at an optimal ratio. We also found that TFAM has a significant advantage over protamine, a nuclear DNA packaging protein, from the viewpoint of transcription efficiency. This result indicates that TFAM can be useful packaging protein for exogenous DNA to achieve mitochondrial transgene expression.

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Access to Document

10.1016/j.fob.2012.06.001