TY - JOUR
T1 - The manner in which DNA is packaged with TFAM has an impact on transcription activation and inhibition
AU - Furukawa, Ryo
AU - Yamada, Yuma
AU - Matsushima, Yuichi
AU - Goto, Yu Ichi
AU - Harashima, Hideyoshi
N1 - Funding Information:
This work was supported, in part by, a Grant-in-Aid for Young Scientists (A) and a Grant-in-Aid for Scientific Research (S) from the Ministry of Education, Culture, Sports, Science and Technology of Japanese Government (MEXT), the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation, Japan (NIBIO). We thank Dr. Milton Feather for his helpful advice in writing the manuscript.
PY - 2012
Y1 - 2012
N2 - For successful mitochondrial transgene expression, an optimal packaging exogenous DNA is an important issue. We report herein on the effects of DNA packaged with mitochondrial transcription factor A (TFAM), which packages mitochondrial DNA (mtDNA), on the transcription process. Our initial findings indicated that the transcription of the TFAM/DNA complex was activated, when the complex was formed at an optimal ratio. We also found that TFAM has a significant advantage over protamine, a nuclear DNA packaging protein, from the viewpoint of transcription efficiency. This result indicates that TFAM can be useful packaging protein for exogenous DNA to achieve mitochondrial transgene expression.
AB - For successful mitochondrial transgene expression, an optimal packaging exogenous DNA is an important issue. We report herein on the effects of DNA packaged with mitochondrial transcription factor A (TFAM), which packages mitochondrial DNA (mtDNA), on the transcription process. Our initial findings indicated that the transcription of the TFAM/DNA complex was activated, when the complex was formed at an optimal ratio. We also found that TFAM has a significant advantage over protamine, a nuclear DNA packaging protein, from the viewpoint of transcription efficiency. This result indicates that TFAM can be useful packaging protein for exogenous DNA to achieve mitochondrial transgene expression.
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U2 - 10.1016/j.fob.2012.06.001
DO - 10.1016/j.fob.2012.06.001
M3 - Article
C2 - 23650593
AN - SCOPUS:84862985087
VL - 2
SP - 145
EP - 150
JO - FEBS Open Bio
JF - FEBS Open Bio
SN - 2211-5463
ER -