The mechanism of reduction of cell-mediated cytotoxicity in neonatally thymectomized mice

H. Kawauchi, K. Taniguchi, Chiharu Kubo, Y. Shimamoto, K. Nomoto

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Abstract

The effect of neonatal thymectomy at various times after birth (Tx-1, Tx-7) on effector and suppressor T cells responsible for cell-mediated cytotoxicity (CMC) for allogeneic antigens was determined. Following in-vitro primary mixed lymphocyte cultures, in the absence of T-cell growth factor (TCGF), alloreactive CMC was not detected in spleen cells of Tx-1 mice, but was detected in spleen cells of Tx-7 mice at as high levels as in those of sham-operated mice. However, in the presence of TCGF, as much alloreactive CMC was detected in spleen cells of Tx-1 mice as in those of Tx-7 mice. Furthermore, TCGF production was not detected in spleen cells of Tx-1 mice but was detected in those of Tx-7 mice. In in-vivo experiments, inhibition of allogeneic tumour growth and CMC in spleen cells showed the same pattern as in in-vitro experiments. These results support the concept that the reduction of CMC in Tx-1 mice might be due to a defect in helper function (TCGF-producing capacity) rather than to a defect in cytotoxic T lymphocytes and/or cytotoxic T lymphocyte precursors. Alloreactive suppressor T cells could not be induced in spleen cells of Tx-1 mice but were induced in spleen cells of Tx-7 mice. Therefore it was suggested that alloreactive suppressor T cells require the presence of the thymus for 7 days after birth in their development.

Original languageEnglish
Pages (from-to)199-205
Number of pages7
JournalImmunology
Volume50
Issue number2
Publication statusPublished - Nov 18 1983

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Spleen
Interleukin-2
Cytotoxic T-Lymphocytes
T-Lymphocytes
Parturition
Thymectomy
Helper-Inducer T-Lymphocytes
Thymus Gland
Lymphocytes
Antigens
Growth
Neoplasms
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Kawauchi, H., Taniguchi, K., Kubo, C., Shimamoto, Y., & Nomoto, K. (1983). The mechanism of reduction of cell-mediated cytotoxicity in neonatally thymectomized mice. Immunology, 50(2), 199-205.

The mechanism of reduction of cell-mediated cytotoxicity in neonatally thymectomized mice. / Kawauchi, H.; Taniguchi, K.; Kubo, Chiharu; Shimamoto, Y.; Nomoto, K.

In: Immunology, Vol. 50, No. 2, 18.11.1983, p. 199-205.

Research output: Contribution to journalArticle

Kawauchi, H, Taniguchi, K, Kubo, C, Shimamoto, Y & Nomoto, K 1983, 'The mechanism of reduction of cell-mediated cytotoxicity in neonatally thymectomized mice', Immunology, vol. 50, no. 2, pp. 199-205.
Kawauchi H, Taniguchi K, Kubo C, Shimamoto Y, Nomoto K. The mechanism of reduction of cell-mediated cytotoxicity in neonatally thymectomized mice. Immunology. 1983 Nov 18;50(2):199-205.
Kawauchi, H. ; Taniguchi, K. ; Kubo, Chiharu ; Shimamoto, Y. ; Nomoto, K. / The mechanism of reduction of cell-mediated cytotoxicity in neonatally thymectomized mice. In: Immunology. 1983 ; Vol. 50, No. 2. pp. 199-205.
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