The mechanism underlying maintenance of the endocochlear potential by the K+ transport system in fibrocytes of the inner ear

Naoko Adachi, Takamasa Yoshida, Fumiaki Nin, Genki Ogata, Soichiro Yamaguchi, Toshihiro Suzuki, Sizuo Komune, Yasuo Hisa, Hiroshi Hibino, Yoshihisa Kurachi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The endocochlear potential (EP) of +80 mV in the scala media, which is indispensable for audition, is controlled by K+ transport across the lateral cochlear wall. This wall includes two epithelial barriers, the syncytium and the marginal cells. The former contains multiple cell types, such as fibrocytes, which are exposed to perilymph on their basolateral surfaces. The apical surfaces of the marginal cells face endolymph. Between the two barriers lies the intrastrial space (IS), an extracellular space with a low K+ concentration ([K+]) and a potential similar to the EP. This intrastrial potential (ISP) dominates the EP and represents the sum of the diffusion potential elicited by a large K+ gradient across the apical surface of the syncytium and the syncytium's potential, which is slightly positive relative to perilymph. Although a K+ transport system in fibrocytes seems to contribute to the EP, the mechanism remains uncertain. We examined the electrochemical properties of the lateral wall of guinea pigs with electrodes sensitive to potential and K+ while perfusing into the perilymph of the scala tympani blockers of Na+,K+-ATPase, the K+ pump thought to be essential to the system. Inhibiting Na+,K+-ATPase barely affected [K+] in the IS but greatly decreased [K+] within the syncytium, reducing the K+ gradient across its apical surface. The treatment hyperpolarized the syncytium only moderately. Consequently, both the ISP and the EP declined. Fibrocytes evidently use the Na+,K+-ATPase to achieve local K+ transport, maintaining the syncytium's high [K+] that is crucial for the K+ diffusion underlying the positive ISP.

Original languageEnglish
Pages (from-to)4459-4472
Number of pages14
JournalJournal of Physiology
Volume591
Issue number18
DOIs
Publication statusPublished - Sep 1 2013

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Inner Ear
Giant Cells
Maintenance
Perilymph
Cochlear Duct
Scala Tympani
Endolymph
Cochlea
Extracellular Space
Hearing
Electrodes
Guinea Pigs
sodium-translocating ATPase

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

The mechanism underlying maintenance of the endocochlear potential by the K+ transport system in fibrocytes of the inner ear. / Adachi, Naoko; Yoshida, Takamasa; Nin, Fumiaki; Ogata, Genki; Yamaguchi, Soichiro; Suzuki, Toshihiro; Komune, Sizuo; Hisa, Yasuo; Hibino, Hiroshi; Kurachi, Yoshihisa.

In: Journal of Physiology, Vol. 591, No. 18, 01.09.2013, p. 4459-4472.

Research output: Contribution to journalArticle

Adachi, N, Yoshida, T, Nin, F, Ogata, G, Yamaguchi, S, Suzuki, T, Komune, S, Hisa, Y, Hibino, H & Kurachi, Y 2013, 'The mechanism underlying maintenance of the endocochlear potential by the K+ transport system in fibrocytes of the inner ear', Journal of Physiology, vol. 591, no. 18, pp. 4459-4472. https://doi.org/10.1113/jphysiol.2013.258046
Adachi, Naoko ; Yoshida, Takamasa ; Nin, Fumiaki ; Ogata, Genki ; Yamaguchi, Soichiro ; Suzuki, Toshihiro ; Komune, Sizuo ; Hisa, Yasuo ; Hibino, Hiroshi ; Kurachi, Yoshihisa. / The mechanism underlying maintenance of the endocochlear potential by the K+ transport system in fibrocytes of the inner ear. In: Journal of Physiology. 2013 ; Vol. 591, No. 18. pp. 4459-4472.
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