The medaka zic1/zic4 mutant provides molecular insights into teleost caudal fin evolution

Yuuta Moriyama, Toru Kawanishi, Ryohei Nakamura, Tatsuya Tsukahara, Kenta Sumiyama, Maximiliano L. Suster, Koichi Kawakami, Atsushi Toyoda, Asao Fujiyama, Yuuri Yasuoka, Yusuke Nagao, Etsuko Sawatari, Atsushi Shimizu, Yuko Wakamatsu, Masahiko Hibi, Masanori Taira, Masataka Okabe, Kiyoshi Naruse, Hisashi Hashimoto, Atsuko ShimadaHiroyuki Takeda

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Teleosts have an asymmetrical caudal fin skeleton formed by the upward bending of the caudal-most portion of the body axis, the ural region [1-3]. This homocercal type of caudal fin ensures powerful and complex locomotion and is regarded as one of the most important innovations for teleosts during adaptive radiation in an aquatic environment [4-6]. However, the mechanisms that create asymmetric caudal fin remain largely unknown. The spontaneous medaka (teleost fish) mutant, Double anal fin (Da), exhibits a unique symmetrical caudal skeleton that resembles the diphycercal type seen in Polypterus and Coelacanth. We performed a detailed analysis of the Da mutant to obtain molecular insight into caudal fin morphogenesis. We first demonstrate that a large transposon, inserted into the enhancer region of the zic1 and zic4 genes (zic1/zic4) in Da, is associated with the mesoderm-specific loss of their transcription. We then show that zic1/zic4 are strongly expressed in the dorsal part of the ural mesenchyme and thereby induce asymmetric caudal fin development in wild-type embryos, whereas their expression is lost in Da. Comparative analysis further indicates that the dorsal mesoderm expression of zic1/zic4 is conserved in teleosts, highlighting the crucial role of zic1/zic4 in caudal fin morphogenesis.

Original languageEnglish
Pages (from-to)601-607
Number of pages7
JournalCurrent Biology
Volume22
Issue number7
DOIs
Publication statusPublished - Apr 10 2012
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Moriyama, Y., Kawanishi, T., Nakamura, R., Tsukahara, T., Sumiyama, K., Suster, M. L., ... Takeda, H. (2012). The medaka zic1/zic4 mutant provides molecular insights into teleost caudal fin evolution. Current Biology, 22(7), 601-607. https://doi.org/10.1016/j.cub.2012.01.063