The microRNA-218~Survivin axis regulates migration, invasion, and lymph node metastasis in cervical cancer

Ryunosuke Kogo, Christine How, Naz Chaudary, Jeff Bruce, Wei Shi, Richard P. Hill, Payam Zahedi, Kenneth W. Yip, Fei Fei Liu

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Cervical cancer is the third most common cancer in women worldwide. In the present study, global microRNA profiling for 79 cervical cancer patient samples led to the identification of miR-218 down-regulation in cervical cancer tissues compared to normal cervical tissues. Lower miR-218 expression was associated significantly with worse overall survival (OS), disease-free survival (DFS), and pelvic/aortic lymph node recurrence. In vitro, miR-218 over-expression decreased clonogenicity, migration, and invasion. Survivin (BIRC5) was subsequently identified as an important cervical cancer target of miR-218 using in silico prediction, mRNA profiling, and quantitative realtime PCR (qRT-PCR). Concordant with miR-218 over-expression, survivin knockdown by siRNA decreased clonogenicity, migration, and invasion. YM155, a small molecule survivin inhibitor, significantly suppressed tumor growth and lymph node metastasis in vivo. Our findings demonstrate that the miR-218~survivin axis inhibits cervical cancer progression by regulating clonogenicity, migration, and invasion, and suggest that the inhibition of survivin could be a potential therapeutic strategy to improve outcome in this disease.

Original languageEnglish
Pages (from-to)1090-1100
Number of pages11
JournalOncotarget
Volume6
Issue number2
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

MicroRNAs
Uterine Cervical Neoplasms
Lymph Nodes
Neoplasm Metastasis
Computer Simulation
Small Interfering RNA
Disease-Free Survival
Neoplasms
Down-Regulation
Recurrence
Polymerase Chain Reaction
Messenger RNA
Survival
Growth
Therapeutics

All Science Journal Classification (ASJC) codes

  • Oncology

Cite this

Kogo, R., How, C., Chaudary, N., Bruce, J., Shi, W., Hill, R. P., ... Liu, F. F. (2015). The microRNA-218~Survivin axis regulates migration, invasion, and lymph node metastasis in cervical cancer. Oncotarget, 6(2), 1090-1100. https://doi.org/10.18632/oncotarget.2836

The microRNA-218~Survivin axis regulates migration, invasion, and lymph node metastasis in cervical cancer. / Kogo, Ryunosuke; How, Christine; Chaudary, Naz; Bruce, Jeff; Shi, Wei; Hill, Richard P.; Zahedi, Payam; Yip, Kenneth W.; Liu, Fei Fei.

In: Oncotarget, Vol. 6, No. 2, 01.01.2015, p. 1090-1100.

Research output: Contribution to journalArticle

Kogo, R, How, C, Chaudary, N, Bruce, J, Shi, W, Hill, RP, Zahedi, P, Yip, KW & Liu, FF 2015, 'The microRNA-218~Survivin axis regulates migration, invasion, and lymph node metastasis in cervical cancer', Oncotarget, vol. 6, no. 2, pp. 1090-1100. https://doi.org/10.18632/oncotarget.2836
Kogo, Ryunosuke ; How, Christine ; Chaudary, Naz ; Bruce, Jeff ; Shi, Wei ; Hill, Richard P. ; Zahedi, Payam ; Yip, Kenneth W. ; Liu, Fei Fei. / The microRNA-218~Survivin axis regulates migration, invasion, and lymph node metastasis in cervical cancer. In: Oncotarget. 2015 ; Vol. 6, No. 2. pp. 1090-1100.
@article{a7dbadcd74f64fefa90d6faeec8832c3,
title = "The microRNA-218~Survivin axis regulates migration, invasion, and lymph node metastasis in cervical cancer",
abstract = "Cervical cancer is the third most common cancer in women worldwide. In the present study, global microRNA profiling for 79 cervical cancer patient samples led to the identification of miR-218 down-regulation in cervical cancer tissues compared to normal cervical tissues. Lower miR-218 expression was associated significantly with worse overall survival (OS), disease-free survival (DFS), and pelvic/aortic lymph node recurrence. In vitro, miR-218 over-expression decreased clonogenicity, migration, and invasion. Survivin (BIRC5) was subsequently identified as an important cervical cancer target of miR-218 using in silico prediction, mRNA profiling, and quantitative realtime PCR (qRT-PCR). Concordant with miR-218 over-expression, survivin knockdown by siRNA decreased clonogenicity, migration, and invasion. YM155, a small molecule survivin inhibitor, significantly suppressed tumor growth and lymph node metastasis in vivo. Our findings demonstrate that the miR-218~survivin axis inhibits cervical cancer progression by regulating clonogenicity, migration, and invasion, and suggest that the inhibition of survivin could be a potential therapeutic strategy to improve outcome in this disease.",
author = "Ryunosuke Kogo and Christine How and Naz Chaudary and Jeff Bruce and Wei Shi and Hill, {Richard P.} and Payam Zahedi and Yip, {Kenneth W.} and Liu, {Fei Fei}",
year = "2015",
month = "1",
day = "1",
doi = "10.18632/oncotarget.2836",
language = "English",
volume = "6",
pages = "1090--1100",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals",
number = "2",

}

TY - JOUR

T1 - The microRNA-218~Survivin axis regulates migration, invasion, and lymph node metastasis in cervical cancer

AU - Kogo, Ryunosuke

AU - How, Christine

AU - Chaudary, Naz

AU - Bruce, Jeff

AU - Shi, Wei

AU - Hill, Richard P.

AU - Zahedi, Payam

AU - Yip, Kenneth W.

AU - Liu, Fei Fei

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Cervical cancer is the third most common cancer in women worldwide. In the present study, global microRNA profiling for 79 cervical cancer patient samples led to the identification of miR-218 down-regulation in cervical cancer tissues compared to normal cervical tissues. Lower miR-218 expression was associated significantly with worse overall survival (OS), disease-free survival (DFS), and pelvic/aortic lymph node recurrence. In vitro, miR-218 over-expression decreased clonogenicity, migration, and invasion. Survivin (BIRC5) was subsequently identified as an important cervical cancer target of miR-218 using in silico prediction, mRNA profiling, and quantitative realtime PCR (qRT-PCR). Concordant with miR-218 over-expression, survivin knockdown by siRNA decreased clonogenicity, migration, and invasion. YM155, a small molecule survivin inhibitor, significantly suppressed tumor growth and lymph node metastasis in vivo. Our findings demonstrate that the miR-218~survivin axis inhibits cervical cancer progression by regulating clonogenicity, migration, and invasion, and suggest that the inhibition of survivin could be a potential therapeutic strategy to improve outcome in this disease.

AB - Cervical cancer is the third most common cancer in women worldwide. In the present study, global microRNA profiling for 79 cervical cancer patient samples led to the identification of miR-218 down-regulation in cervical cancer tissues compared to normal cervical tissues. Lower miR-218 expression was associated significantly with worse overall survival (OS), disease-free survival (DFS), and pelvic/aortic lymph node recurrence. In vitro, miR-218 over-expression decreased clonogenicity, migration, and invasion. Survivin (BIRC5) was subsequently identified as an important cervical cancer target of miR-218 using in silico prediction, mRNA profiling, and quantitative realtime PCR (qRT-PCR). Concordant with miR-218 over-expression, survivin knockdown by siRNA decreased clonogenicity, migration, and invasion. YM155, a small molecule survivin inhibitor, significantly suppressed tumor growth and lymph node metastasis in vivo. Our findings demonstrate that the miR-218~survivin axis inhibits cervical cancer progression by regulating clonogenicity, migration, and invasion, and suggest that the inhibition of survivin could be a potential therapeutic strategy to improve outcome in this disease.

UR - http://www.scopus.com/inward/record.url?scp=84921825261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84921825261&partnerID=8YFLogxK

U2 - 10.18632/oncotarget.2836

DO - 10.18632/oncotarget.2836

M3 - Article

C2 - 25473903

AN - SCOPUS:84921825261

VL - 6

SP - 1090

EP - 1100

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 2

ER -