The microtubule destabilizer stathmin mediates the development of dendritic arbors in neuronal cells

Noriaki Ohkawa, Kazuko Fujitani, Eri Tokunaga, Shigeki Furuya, Kaoru Inokuchi

Research output: Contribution to journalArticle

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Abstract

The regulation of microtubule dynamics is important for the appropriate arborization of neuronal dendrites during development, which in turn is critical for the formation of functional neural networks. Here we show that stathmin, a microtubule destabilizing factor, is downregulated at both the expression and activity levels during cerebellar development, and this down-regulation contributes to dendritic arborization. Stathmin overexpression drastically limited the dendritic growth of cultured Purkinje cells. The stathmin activity was suppressed by neural activity and CaMKII-dependent phosphorylation at Ser16, which led to dendritic arborization. Stathmin phosphorylation at Ser16 was mediated by the activation of voltage-gated calcium channels and metabotropic glutamate receptor 1. Although overexpression of SCG1O, a member of the stathmin family, also limited the dendritic arborization, SCG10 did not mediate the CaMKII regulation of dendritic development. These results suggest that calcium elevation activates CaMKII, which in turn phosphorylates stathmin at Ser16 to stabilize dendritic microtubules. siRNA knockdown of endogenous stathmin significantly reduced dendritic growth in Purkinje cells. Thus, these data suggest that proper regulation of stathmin activity is a key factor for controlling the dendritic microtubule dynamics that are important for neuronal development.

Original languageEnglish
Pages (from-to)1447-1456
Number of pages10
JournalJournal of Cell Science
Volume120
Issue number8
DOIs
Publication statusPublished - Apr 15 2007

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Stathmin
Microtubules
Neuronal Plasticity
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Purkinje Cells
Down-Regulation
Phosphorylation
Calcium Channels
Growth
Small Interfering RNA
Cultured Cells
Calcium

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

The microtubule destabilizer stathmin mediates the development of dendritic arbors in neuronal cells. / Ohkawa, Noriaki; Fujitani, Kazuko; Tokunaga, Eri; Furuya, Shigeki; Inokuchi, Kaoru.

In: Journal of Cell Science, Vol. 120, No. 8, 15.04.2007, p. 1447-1456.

Research output: Contribution to journalArticle

Ohkawa, Noriaki ; Fujitani, Kazuko ; Tokunaga, Eri ; Furuya, Shigeki ; Inokuchi, Kaoru. / The microtubule destabilizer stathmin mediates the development of dendritic arbors in neuronal cells. In: Journal of Cell Science. 2007 ; Vol. 120, No. 8. pp. 1447-1456.
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