The MMP activity in developing rat molar roots and incisors demonstrated by in situ zymography

Ichiro Sakuraba, Junko Hatakeyama, Yuji Hatakeyama, Ichiro Takahashi, Hideaki Mayanagi, Yasuyuki Sasano

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Matrix metalloproteinases (MMPs) have been expressed during root development and periodontal tissue formation, whereas it is not known if these MMP molecules are enzymatically active to degrade the extracellular matrices (ECMs). The present study was designed to investigate the gelatinolytic and collagenolytic activity in rat molar root and incisor development. Three-week old rat mandibles were frozen and cut without fixation or decalcification and processed for in situ zymography using substrates gelatin and collagen. The enzymatic activity was assessed according to the intensity of fluorescence due to the lysis of the substrates. Odontoblasts, predentin, cementum, bone and the enamel matrix showed the high activity. The present study demonstrated MMP activity in calcified tissues using in situ zymography for the first time and the possible involvement of the MMP activity in molar root and incisor development and periodontal tissue formation.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalJournal of Molecular Histology
Volume37
Issue number1-2
DOIs
Publication statusPublished - Jan 1 2006
Externally publishedYes

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Incisor
Matrix Metalloproteinases
Odontoblasts
Dental Cementum
Bone Matrix
Gelatin
Dental Enamel
Mandible
Extracellular Matrix
Collagen
Fluorescence

All Science Journal Classification (ASJC) codes

  • Histology
  • Physiology
  • Cell Biology

Cite this

The MMP activity in developing rat molar roots and incisors demonstrated by in situ zymography. / Sakuraba, Ichiro; Hatakeyama, Junko; Hatakeyama, Yuji; Takahashi, Ichiro; Mayanagi, Hideaki; Sasano, Yasuyuki.

In: Journal of Molecular Histology, Vol. 37, No. 1-2, 01.01.2006, p. 87-93.

Research output: Contribution to journalArticle

Sakuraba, Ichiro ; Hatakeyama, Junko ; Hatakeyama, Yuji ; Takahashi, Ichiro ; Mayanagi, Hideaki ; Sasano, Yasuyuki. / The MMP activity in developing rat molar roots and incisors demonstrated by in situ zymography. In: Journal of Molecular Histology. 2006 ; Vol. 37, No. 1-2. pp. 87-93.
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