The molecular clock regulates circadian transcription of tissue factor gene

Katsutaka Oishi, Koyanagi Satoru, Naoki Ohkura

Research output: Contribution to journalArticle

Abstract

Tissue factor (TF) is involved in endotoxin-induced inflammation and mortality. We found that the circadian expression of TF mRNA, which peaked at the day to night transition (activity onset), was damped in the liver of Clock mutant mice. Luciferase reporter and chromatin immunoprecipitation analyses using embryonic fibroblasts derived from wild-type or Clock mutant mice showed that CLOCK is involved in transcription of the TF gene. Furthermore, the results of real-time luciferase reporter experiments revealed that the circadian expression of TF mRNA is regulated by clock molecules through a cell-autonomous mechanism via an E-box element located in the promoter region.

Original languageEnglish
Pages (from-to)332-335
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume431
Issue number2
DOIs
Publication statusPublished - Feb 8 2013

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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