Abstract
Tissue factor (TF) is involved in endotoxin-induced inflammation and mortality. We found that the circadian expression of TF mRNA, which peaked at the day to night transition (activity onset), was damped in the liver of Clock mutant mice. Luciferase reporter and chromatin immunoprecipitation analyses using embryonic fibroblasts derived from wild-type or Clock mutant mice showed that CLOCK is involved in transcription of the TF gene. Furthermore, the results of real-time luciferase reporter experiments revealed that the circadian expression of TF mRNA is regulated by clock molecules through a cell-autonomous mechanism via an E-box element located in the promoter region.
| Original language | English |
|---|---|
| Pages (from-to) | 332-335 |
| Number of pages | 4 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 431 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 8 2013 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
The molecular clock regulates circadian transcription of tissue factor gene. / Oishi, Katsutaka; Satoru, Koyanagi; Ohkura, Naoki.
In: Biochemical and Biophysical Research Communications, Vol. 431, No. 2, 08.02.2013, p. 332-335.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The molecular clock regulates circadian transcription of tissue factor gene
AU - Oishi, Katsutaka
AU - Satoru, Koyanagi
AU - Ohkura, Naoki
PY - 2013/2/8
Y1 - 2013/2/8
N2 - Tissue factor (TF) is involved in endotoxin-induced inflammation and mortality. We found that the circadian expression of TF mRNA, which peaked at the day to night transition (activity onset), was damped in the liver of Clock mutant mice. Luciferase reporter and chromatin immunoprecipitation analyses using embryonic fibroblasts derived from wild-type or Clock mutant mice showed that CLOCK is involved in transcription of the TF gene. Furthermore, the results of real-time luciferase reporter experiments revealed that the circadian expression of TF mRNA is regulated by clock molecules through a cell-autonomous mechanism via an E-box element located in the promoter region.
AB - Tissue factor (TF) is involved in endotoxin-induced inflammation and mortality. We found that the circadian expression of TF mRNA, which peaked at the day to night transition (activity onset), was damped in the liver of Clock mutant mice. Luciferase reporter and chromatin immunoprecipitation analyses using embryonic fibroblasts derived from wild-type or Clock mutant mice showed that CLOCK is involved in transcription of the TF gene. Furthermore, the results of real-time luciferase reporter experiments revealed that the circadian expression of TF mRNA is regulated by clock molecules through a cell-autonomous mechanism via an E-box element located in the promoter region.
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UR - http://www.scopus.com/inward/citedby.url?scp=84873460817&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2012.12.098
DO - 10.1016/j.bbrc.2012.12.098
M3 - Article
C2 - 23291174
AN - SCOPUS:84873460817
VL - 431
SP - 332
EP - 335
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -