The mouse embryo autonomously acquires anterior-posterior polarity at implantation

Katsuyoshi Takaoka, Masamichi Yamamoto, Hidetaka Shiratori, Chikara Meno, Janet Rossant, Yukio Saijoh, Hiroshi Hamada

Research output: Contribution to journalArticlepeer-review

96 Citations (Scopus)

Abstract

The earliest recognizable sign of patterning of the mouse embryo along the anteroposterior (A-P) axis is the migration of the distal visceral endoderm (DVE) toward the future anterior side. Here we report an asymmetry in the mouse embryo at an unexpectedly early stage. The gene for Lefty1, a Nodal antagonist that influences the direction of DVE migration, was found to be asymmetrically expressed in the primitive endoderm of the implanting blastocyst. Lefty1 expression begins randomly in the inner cell mass (ICM) of the blastocyst but is regionalized to one side of the tilted ICM shortly after implantation. Asymmetric expression of Lefty1 can be established by in vitro culture, indicating that it does not require interaction with the uterus. The asymmetric Lefty1 expression is induced by Nodal signaling, although Nodal and genes for its effectors are expressed symmetrically. This asymmetry in molecular patterning of the mouse embryo pushes back the origin of the A-P body axis to the peri-implantation stage.

Original languageEnglish
Pages (from-to)451-459
Number of pages9
JournalDevelopmental Cell
Volume10
Issue number4
DOIs
Publication statusPublished - Apr 1 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Developmental Biology

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