The occurrence of unusual smooth muscle bundles expressing α-smooth muscle actin in human intestinal atresia

Kouji Masumoto, Sachiyo Suita, Tomoaki Taguchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background/Purpose: Intestinal dysmotility is an important problem in the postoperative management of patients with intestinal atresia (IA). Changes in the intramural components have so far been histochemically and immunohistochemically examined in both the proximal and distal segments of IA, but no detailed analysis of the muscular elements has been performed. The aim of this study was to carefully examine any alterations in the muscular elements in the intestines from patients with IA. Methods: Resected intestines were obtained from 6 patients with ileal atresia, 4 patients with jejunal atresia, and 3 controls without gastrointestinal diseases obtained by autopsy (congenital diaphragmatic hernia). All specimens were immunochemically stained with a monoclonal antibody to α-smooth muscle actin (α-SMA) as a smooth muscle marker. Results: In the normal small intestine, almost all the enteric smooth musculature were positive for α-SMA antiserum, except for the bulk of the circular musculature. In the proximal segments of all cases with IA, a reduced staining intensity for α-SMA was observed mainly in the severely hypertrophic muscle layers. In addition, some bundles of smooth muscle also were located in the submucosal connective tissue near the border of the innermost layer of the circular musculature, in which large amounts of smooth muscle fibers extended occasionally from the innermost layer of the circular musculature to the muscularis mucosae in the proximal segments of 4 cases. In the distal segments of IA, the distribution of α-SMA-positive smooth muscle fibers was similar to that in the control intestines, excluding mild to moderate hypertrophy of the muscular layers. Conclusions: Both severe hypertrophy and a reduced immunoreactivity for α-SMA were observed in the circular muscle layer of the proximal segments. In addition, the occurrence of α-SMA-positive abnormal smooth muscle fibers was recognized in the submucosal layers of the proximal segments, thus, suggesting a delay in the intestinal muscular formation or a regressive reaction secondary to dilatation. These muscular alterations in the proximal segments might be considered to contribute to the postoperative intestinal dysmotility in IA cases.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalJournal of Pediatric Surgery
Volume38
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

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Intestinal Atresia
Smooth Muscle
Actins
Intestines
Hypertrophy
Muscles
Gastrointestinal Diseases
Connective Tissue
Small Intestine
Immune Sera
Dilatation
Autopsy
Mucous Membrane
Monoclonal Antibodies
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pediatrics, Perinatology, and Child Health

Cite this

The occurrence of unusual smooth muscle bundles expressing α-smooth muscle actin in human intestinal atresia. / Masumoto, Kouji; Suita, Sachiyo; Taguchi, Tomoaki.

In: Journal of Pediatric Surgery, Vol. 38, No. 2, 01.02.2003, p. 161-166.

Research output: Contribution to journalArticle

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