The och1 mutant of schizosaccharomyces pombe produces galactosylated core structures of n-linked oligosaccharides

Takao Ohashi, Yuka Ikeda, Naotaka Tanaka, Shin Ichi Nakakita, Shunji Natsuka, Yuko Giga-Hama, Kaoru Takegawa

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19 Citations (Scopus)

Abstract

Unlike the budding yeast Saccharomyces cerevisiae, the fission yeast Schizosaccharomyces pombe synthesizes large outer chains on the N-linked oligosaccharides that consist mainly of D-Gal and D-Man residues. The fission yeast och1+ gene product has γ1,6-mannosyltransferase activity, and Och1p is the key enzyme in the initiation of outer chain elongation. Although the in vitro substrate specificity of S. pombe Och1p has been reported (Yoko-o et al., FEBS Lett., 489, 75-80 (2001)), the structure of the N-linked oligosaccharides of och1δ cells has not been investigated. In this study, we report a structural analysis of S. pombe N-linked oligosaccharides. Lectin blot analysis indicated that galactose residues were attached to the cell surface glycoproteins of the och1δ cells. We conducted a structural analysis of pyridylaminated N-linked oligosaccharides prepared from galactomannoproteins by HPLC and 1H NMR. These analyses revealed that the N-linked oligosaccharides of the och1δ cells displayed heterogeneity in the glycan consisting of Hex11-15GlcNAc2. The structural heterogeneity arose mainly from the addition of γ1,2- and γ1,3-Gal residues to the Man9GlcNAc2 core structure.

Original languageEnglish
Pages (from-to)407-414
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number2
DOIs
Publication statusPublished - May 7 2009

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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