The Overexpression of Twinkle Helicase Ameliorates the Progression of Cardiac Fibrosis and Heart Failure in Pressure Overload Model in Mice

Atsushi Tanaka, Tomomi Ide, Takeo Fujino, Ken Onitsuka, Masataka Ikeda, Takako Takehara, Yuko Hata, Emil Ylikallio, Henna Tyynismaa, Anu Suomalainen, Kenji Sunagawa

Research output: Contribution to journalArticle

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Abstract

Myocardial mitochondrial DNA (mtDNA) copy number decreases in heart failure. In post-myocardial infarction mice, increasing mtDNA copy number by overexpressing mitochondrial transcription factor attenuates mtDNA deficiency and ameliorates pathological remodeling thereby markedly improving survival. However, the functional significance of increased mtDNA copy number in hypertensive heart disease remains unknown. We addressed this question using transgenic mice that overexpress Twinkle helicase (Twinkle; Tg), the mtDNA helicase, and examined whether Twinkle overexpression protects the heart from left ventricular (LV) remodeling and failure after pressure overload created by transverse aortic constriction (TAC). Twinkle overexpression increased mtDNA copy number by 2.2±0.1-fold. Heart weight, LV diastolic volume and wall thickness were comparable between Tg and wild type littermates (WT) at 28 days after TAC operation. LV end-diastolic pressure increased in WT after TAC (8.6±2.8 mmHg), and this increase was attenuated in Tg (4.6±2.6 mmHg). Impaired LV fractional shortening after TAC operation was also suppressed in Tg, as measured by echocardiography (WT: 16.2±7.2% vs Tg: 20.7±6.2%). These LV functional improvements were accompanied by a decrease in interstitial fibrosis (WT: 10.6±1.1% vs Tg: 3.0±0.6%). In in vitro studies, overexpressing Twinkle using an adenovirus vector in cultured cardiac fibroblasts significantly suppressed mRNA of collagen 1a, collagen 3a and connective tissue growth factor, and angiotensin II-induced transforming growth factor β1 expression. The findings suggest that Twinkle overexpression prevents LV function deterioration. In conclusion, Twinkle overexpression increases mtDNA copy number and ameliorates the progression of LV fibrosis and heart failure in a mouse pressure overload model. Increasing mtDNA copy number by Twinkle overexpression could be a novel therapeutic strategy for hypertensive heart disease.

Original languageEnglish
Article numbere67642
JournalPloS one
Volume8
Issue number6
DOIs
Publication statusPublished - Jun 28 2013

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heart failure
fibrosis
Mitochondrial DNA
Fibrosis
mitochondrial DNA
Heart Failure
animal models
Pressure
Constriction
heart diseases
collagen
Heart Diseases
mice
Collagen
heart
DNA helicases
DNA Helicases
Connective Tissue Growth Factor
Echocardiography
transforming growth factors

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

The Overexpression of Twinkle Helicase Ameliorates the Progression of Cardiac Fibrosis and Heart Failure in Pressure Overload Model in Mice. / Tanaka, Atsushi; Ide, Tomomi; Fujino, Takeo; Onitsuka, Ken; Ikeda, Masataka; Takehara, Takako; Hata, Yuko; Ylikallio, Emil; Tyynismaa, Henna; Suomalainen, Anu; Sunagawa, Kenji.

In: PloS one, Vol. 8, No. 6, e67642, 28.06.2013.

Research output: Contribution to journalArticle

Tanaka, A, Ide, T, Fujino, T, Onitsuka, K, Ikeda, M, Takehara, T, Hata, Y, Ylikallio, E, Tyynismaa, H, Suomalainen, A & Sunagawa, K 2013, 'The Overexpression of Twinkle Helicase Ameliorates the Progression of Cardiac Fibrosis and Heart Failure in Pressure Overload Model in Mice', PloS one, vol. 8, no. 6, e67642. https://doi.org/10.1371/journal.pone.0067642
Tanaka, Atsushi ; Ide, Tomomi ; Fujino, Takeo ; Onitsuka, Ken ; Ikeda, Masataka ; Takehara, Takako ; Hata, Yuko ; Ylikallio, Emil ; Tyynismaa, Henna ; Suomalainen, Anu ; Sunagawa, Kenji. / The Overexpression of Twinkle Helicase Ameliorates the Progression of Cardiac Fibrosis and Heart Failure in Pressure Overload Model in Mice. In: PloS one. 2013 ; Vol. 8, No. 6.
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abstract = "Myocardial mitochondrial DNA (mtDNA) copy number decreases in heart failure. In post-myocardial infarction mice, increasing mtDNA copy number by overexpressing mitochondrial transcription factor attenuates mtDNA deficiency and ameliorates pathological remodeling thereby markedly improving survival. However, the functional significance of increased mtDNA copy number in hypertensive heart disease remains unknown. We addressed this question using transgenic mice that overexpress Twinkle helicase (Twinkle; Tg), the mtDNA helicase, and examined whether Twinkle overexpression protects the heart from left ventricular (LV) remodeling and failure after pressure overload created by transverse aortic constriction (TAC). Twinkle overexpression increased mtDNA copy number by 2.2±0.1-fold. Heart weight, LV diastolic volume and wall thickness were comparable between Tg and wild type littermates (WT) at 28 days after TAC operation. LV end-diastolic pressure increased in WT after TAC (8.6±2.8 mmHg), and this increase was attenuated in Tg (4.6±2.6 mmHg). Impaired LV fractional shortening after TAC operation was also suppressed in Tg, as measured by echocardiography (WT: 16.2±7.2{\%} vs Tg: 20.7±6.2{\%}). These LV functional improvements were accompanied by a decrease in interstitial fibrosis (WT: 10.6±1.1{\%} vs Tg: 3.0±0.6{\%}). In in vitro studies, overexpressing Twinkle using an adenovirus vector in cultured cardiac fibroblasts significantly suppressed mRNA of collagen 1a, collagen 3a and connective tissue growth factor, and angiotensin II-induced transforming growth factor β1 expression. The findings suggest that Twinkle overexpression prevents LV function deterioration. In conclusion, Twinkle overexpression increases mtDNA copy number and ameliorates the progression of LV fibrosis and heart failure in a mouse pressure overload model. Increasing mtDNA copy number by Twinkle overexpression could be a novel therapeutic strategy for hypertensive heart disease.",
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