The perforin mediated apoptotic pathway in lung injury and fibrosis

H. Miyazaki, K. Kuwano, K. Yoshida, T. Maeyama, M. Yoshimi, M. Fujita, N. Hagimoto, R. Yoshida, Y. Nakanishi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Aims: The perforin mediated pathway is the major pathway of cytotoxicity induced by activated T cells and natural killer cells, and may be involved in the development of pulmonary fibrosis. Methods: Perforin and granzyme B expression were examined in idiopathic pulmonary fibrosis by means of immunohistochemistry, and perforin knockout mice were used to examine whether or not perforin mediated cytotoxicity participates in the pathophysiology of bleomycin induced pneumopathy. Results: Perforin and granzyme B expression were upregulated in infiltrating lymphocytes in lung tissue from patients with idiopathic pulmonary fibrosis compared with normal lung parenchyma. Perforin and granzyme B expression were upregulated predominantly in infiltrating mononuclear cells after bleomycin instillation in wild-type mice. Although the development of bleomycin induced pneumopathy was not completely prevented, the pathological grade of inflammation and fibrosis, and the number of apoptotic cells in lung tissue, were significantly decreased in perforin knockout mice compared with wild-type mice. Conclusions: These results suggest that perforin mediated apoptosis may be associated with the pathophysiology of lung injury and fibrosis.

Original languageEnglish
Pages (from-to)1292-1298
Number of pages7
JournalJournal of Clinical Pathology
Volume57
Issue number12
DOIs
Publication statusPublished - Dec 1 2004

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Perforin
Lung Injury
Fibrosis
Idiopathic Pulmonary Fibrosis
Knockout Mice
Lung
Pulmonary Fibrosis
Bleomycin
Natural Killer Cells
Cell Count
Immunohistochemistry
Lymphocytes
Apoptosis
Inflammation
T-Lymphocytes
perforin-granzyme B

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Miyazaki, H., Kuwano, K., Yoshida, K., Maeyama, T., Yoshimi, M., Fujita, M., ... Nakanishi, Y. (2004). The perforin mediated apoptotic pathway in lung injury and fibrosis. Journal of Clinical Pathology, 57(12), 1292-1298. https://doi.org/10.1136/jcp.2003.015495

The perforin mediated apoptotic pathway in lung injury and fibrosis. / Miyazaki, H.; Kuwano, K.; Yoshida, K.; Maeyama, T.; Yoshimi, M.; Fujita, M.; Hagimoto, N.; Yoshida, R.; Nakanishi, Y.

In: Journal of Clinical Pathology, Vol. 57, No. 12, 01.12.2004, p. 1292-1298.

Research output: Contribution to journalArticle

Miyazaki, H, Kuwano, K, Yoshida, K, Maeyama, T, Yoshimi, M, Fujita, M, Hagimoto, N, Yoshida, R & Nakanishi, Y 2004, 'The perforin mediated apoptotic pathway in lung injury and fibrosis', Journal of Clinical Pathology, vol. 57, no. 12, pp. 1292-1298. https://doi.org/10.1136/jcp.2003.015495
Miyazaki H, Kuwano K, Yoshida K, Maeyama T, Yoshimi M, Fujita M et al. The perforin mediated apoptotic pathway in lung injury and fibrosis. Journal of Clinical Pathology. 2004 Dec 1;57(12):1292-1298. https://doi.org/10.1136/jcp.2003.015495
Miyazaki, H. ; Kuwano, K. ; Yoshida, K. ; Maeyama, T. ; Yoshimi, M. ; Fujita, M. ; Hagimoto, N. ; Yoshida, R. ; Nakanishi, Y. / The perforin mediated apoptotic pathway in lung injury and fibrosis. In: Journal of Clinical Pathology. 2004 ; Vol. 57, No. 12. pp. 1292-1298.
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