The phylogenetic position of the endemic flat-needle pine Pinus krempfii (Pinaceae) from Vietnam, based on PCR-RFLP analysis of chloroplast DNA

Xiao Ru Wang, Alfred Szmidt, Hoang Nghia Nguyên

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Pinus krempfii is morphologically very unique as compared to other Pinus species by having flat leaf-like needles. Its taxonomic position has been problematic ever since its discovery. In this study, an attempt was made to infer the taxonomic status of P. krempfii through restriction fragment length polymorphism analysis of 12 PCR amplified chloroplast (cp) DNA regions. Phylogenetic analysis was conducted using 10 representatives of the two Pinus subgenera: Strobus and Pinus. In addition, to infer the position of P. krempfii in Pinaceae in relation with other genera, 14 representatives of eight additional genera were included in the analysis. Our cpDNA-based results indicate that: 1) P. krempfii clearly belongs to the genus Pinus. This result does not favour the creation of a new genus Ducampopinus in Pinaceae for this taxon. 2) Within the genus Pinus, P. krempfii is more allied with species in subgenus Strobus and differs distinctly from species in subgenus Pinus. 3) Despite the similarity in certain morphological and anatomical leaf and wood characters to Keteleeria and Pseudolarix, the cpDNA data do not support the hypothesis for close relationship between P. krempfii and these two genera.

Original languageEnglish
Pages (from-to)21-36
Number of pages16
JournalPlant Systematics and Evolution
Volume220
Issue number1-2
DOIs
Publication statusPublished - Jan 1 2000

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conifer needles
Pinaceae
chloroplast DNA
chloroplast
Vietnam
restriction fragment length polymorphism
Pinus
phylogenetics
DNA
phylogeny
new genus
polymorphism
Pseudolarix
analysis
leaves

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Cite this

The phylogenetic position of the endemic flat-needle pine Pinus krempfii (Pinaceae) from Vietnam, based on PCR-RFLP analysis of chloroplast DNA. / Wang, Xiao Ru; Szmidt, Alfred; Nguyên, Hoang Nghia.

In: Plant Systematics and Evolution, Vol. 220, No. 1-2, 01.01.2000, p. 21-36.

Research output: Contribution to journalArticle

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