The plant vacuolar sorting receptor AtELP is involved in transport of NH2-terminal propeptide-containing vacuolar proteins in Arabidopsis thaliana

Sharif U. Ahmed, Enrique Rojo, Valentina Kovaleva, Sridhar Venkataraman, James E. Dombrowski, Ken Matsuoka, Natasha V. Raikhel

Research output: Contribution to journalArticle

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Abstract

Many soluble plant vacuolar proteins are sorted away from secreted proteins into small vesicles at the trans-Golgi network by transmembrane cargo receptors. Cleavable vacuolar sorting signals include the NH2-terminal propeptide (NTPP) present in sweet potato sporamin (Spo) and the COOH- terminal propeptide (CTPP) present in barley lectin (BL). These two proteins have been found to be transported by different mechanisms to the vacuole. We examined the ability of the vacuolar cargo receptor AtELP to interact with the sorting signals of heterologous and endogenous plant vacuolar proteins in mediating vacuolar transport in Arabidopsis thaliana. AtELP extracted from microsomes was found to interact with the NTPPs of barley aleurain and Spo, but not with the CTPPs of BL or tobacco chitinase, in a pH-dependent and sequence-specific manner. In addition, EM studies revealed the colocalization of AtELP with NTPP-Spo at the Golgi apparatus, but not with BL-CTPP in roots of transgenic Arabidopsis plants. Further, we found that AtELP interacts in a similar manner with the NTPP of the endogenous vacuolar protein AtALEU (Arabidopsis thaliana Aleu), a protein highly homologous to barley aleurain. We hypothesize that AtELP functions as a vacuolar sorting receptor involved in the targeting of NTPP-, but not CTPP-containing proteins in Arabidopsis.

Original languageEnglish
Pages (from-to)1335-1344
Number of pages10
JournalJournal of Cell Biology
Volume149
Issue number7
DOIs
Publication statusPublished - Jun 26 2000
Externally publishedYes

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Arabidopsis Proteins
Plant Proteins
Hordeum
Arabidopsis
Ipomoea batatas
trans-Golgi Network
Chitinases
Proteins
Genetically Modified Plants
Golgi Apparatus
Microsomes
Vacuoles
Tobacco
barley lectin
aleurain

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

The plant vacuolar sorting receptor AtELP is involved in transport of NH2-terminal propeptide-containing vacuolar proteins in Arabidopsis thaliana. / Ahmed, Sharif U.; Rojo, Enrique; Kovaleva, Valentina; Venkataraman, Sridhar; Dombrowski, James E.; Matsuoka, Ken; Raikhel, Natasha V.

In: Journal of Cell Biology, Vol. 149, No. 7, 26.06.2000, p. 1335-1344.

Research output: Contribution to journalArticle

Ahmed, Sharif U. ; Rojo, Enrique ; Kovaleva, Valentina ; Venkataraman, Sridhar ; Dombrowski, James E. ; Matsuoka, Ken ; Raikhel, Natasha V. / The plant vacuolar sorting receptor AtELP is involved in transport of NH2-terminal propeptide-containing vacuolar proteins in Arabidopsis thaliana. In: Journal of Cell Biology. 2000 ; Vol. 149, No. 7. pp. 1335-1344.
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