TY - JOUR
T1 - The positive relationship between γH2AX and PD-L1 expression in lung squamous cell carcinoma
AU - Osoegawa, Atsushi
AU - Hiraishi, Hitomi
AU - Hashimoto, Takafumi
AU - Takumi, Yohei
AU - Abe, Miyuki
AU - Takeuchi, Hideya
AU - Miyawaki, Michiyo
AU - Okamoto, Tatsuro
AU - Sugio, Kenji
N1 - Funding Information:
The Authors would like to thank Ms. Yuko Hono for technical assistance and Mr. Brian Quinn for his critical comments on the manuscript. This research is partly funded by Takeda Science Foundation (AO) and OITA Cancer Research Foundation (AO).
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Background/Aim: Lung squamous cell carcinoma often arises from precancerous lesions where alterations in tumor suppressor genes and subsequent chromosomal instability are often observed due to carcinogen exposure. These tumors are often immunogenic; as such, immune checkpoint inhibitors are a promising therapeutic option. We hypothesized that the DNA damage response in tumor cells induces an immune response, thereby up-regulating programmed death-ligand 1 (PD-L1) expression on tumor cells, which in turn sensitizes them to anti-PD-1 therapy. Patients and Methods: An immunohistochemical analysis was performed in 41 consecutive lung squamous cell carcinoma patients who underwent surgery at our institution between April 2013 and March 2014. Results: The analysis revealed a high PD-L1 expression in 15 patients (37%) (p=0.028). The PD-L1 expression was positively associated with the nuclear γH2AX expression (p=0.02), that was confirmed by immunofluorescent staining. Conclusion: Our findings demonstrate that nuclear γH2AX expression is positively associated with the PD-L1 expression in lung squamous cell carcinoma.
AB - Background/Aim: Lung squamous cell carcinoma often arises from precancerous lesions where alterations in tumor suppressor genes and subsequent chromosomal instability are often observed due to carcinogen exposure. These tumors are often immunogenic; as such, immune checkpoint inhibitors are a promising therapeutic option. We hypothesized that the DNA damage response in tumor cells induces an immune response, thereby up-regulating programmed death-ligand 1 (PD-L1) expression on tumor cells, which in turn sensitizes them to anti-PD-1 therapy. Patients and Methods: An immunohistochemical analysis was performed in 41 consecutive lung squamous cell carcinoma patients who underwent surgery at our institution between April 2013 and March 2014. Results: The analysis revealed a high PD-L1 expression in 15 patients (37%) (p=0.028). The PD-L1 expression was positively associated with the nuclear γH2AX expression (p=0.02), that was confirmed by immunofluorescent staining. Conclusion: Our findings demonstrate that nuclear γH2AX expression is positively associated with the PD-L1 expression in lung squamous cell carcinoma.
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U2 - 10.21873/invivo.11221
DO - 10.21873/invivo.11221
M3 - Article
C2 - 29275316
AN - SCOPUS:85039704087
SN - 0258-851X
VL - 32
SP - 171
EP - 177
JO - In Vivo
JF - In Vivo
IS - 1
ER -